FAS antibody (Fas (TNF Receptor Superfamily, Member 6))

Details for Product anti-FAS Antibody No. ABIN965824, Supplier: Log in to see
Antigen
  • AI196731
  • APO1
  • APT1
  • CD95
  • TNFR6
  • Tnfrsf6
  • lpr
  • TNFRSF6
  • ALPS1A
  • APO-1
  • FAS1
  • FASTM
  • A630082H08Rik
  • FAS
  • Fas (TNF receptor superfamily member 6)
  • Fas (TNF receptor superfamily, member 6)
  • Fas cell surface death receptor
  • fatty acid synthase
  • Fas
  • FAS
  • Fasn
Alternatives
anti-Human FAS antibody for Activation
Reactivity
Human
640
112
99
15
11
10
6
6
3
3
3
3
2
1
Host
Mouse
413
298
23
5
4
3
3
Clonality (Clone)
Monoclonal ()
Conjugate
This FAS antibody is un-conjugated
73
63
51
29
15
8
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7
5
5
5
4
4
4
4
4
4
2
2
2
2
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2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC)
333
311
207
144
112
84
60
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52
46
23
18
12
7
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6
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3
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2
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2
2
1
1
1
1
1
Options
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Immunogen Purified recombinant Fas antigen.
Clone B500 (B-R18)
Isotype IgG1
Specificity This antibody reacts with CD95, a 40-45kD cell surface glycoprotein containing 8kD of N-glycosidic-linked polysaccharide and that is expressed by the CD45RAlow and CD45ROhigh subset of memory T cells.
Purification Concentrated.
Alternative Name CD95 (FAS Antibody Abstract)
Gene ID 355
Research Area Immunology, Innate Immunity, Cytokines, CD Antigens, Apoptosis/Necrosis, Death Receptors, Cancer, Growth Factors, Surface Receptors of Immune Cells
Pathways p53 Signaling, Apoptosis, TCR Signaling, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Application Notes Immunohistochemistry: 1:50-1:100.
Flow cytometry: 1:150-1:300.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using with formalin-fixed paraffin embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.
Comment

Recommended Positive Control: Tonsil, Spleen, Thymus.

Restrictions For Research Use only
Format Liquid
Concentration 0.3 mg/ml.
Buffer 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Debatin, Goldman, Waldmann, Krammer: "APO-1-induced apoptosis of leukemia cells from patients with adult T-cell leukemia." in: Blood, Vol. 81, Issue 11, pp. 2972-7, 1993 (PubMed).

Möller, Henne, Leithäuser, Eichelmann, Schmidt, Brüderlein, Dhein, Krammer et al.: "Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells and in follicle center and mediastinal ..." in: Blood, Vol. 81, Issue 8, pp. 2067-75, 1993 (PubMed).

Oehm, Behrmann, Falk, Pawlita, Maier, Klas, Li-Weber, Richards, Dhein, Trauth: "Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen." in: The Journal of biological chemistry, Vol. 267, Issue 15, pp. 10709-15, 1992 (PubMed).

Itoh, Yonehara, Ishii, Yonehara, Mizushima, Sameshima, Hase, Seto, Nagata: "The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis." in: Cell, Vol. 66, Issue 2, pp. 233-43, 1991 (PubMed).

Trauth, Klas, Peters, Matzku, Möller, Falk, Debatin, Krammer: "Monoclonal antibody-mediated tumor regression by induction of apoptosis." in: Science (New York, N.Y.), Vol. 245, Issue 4915, pp. 301-5, 1989 (PubMed).