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Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer75) antibody

Antigen

Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A)
Synonyms CDC25A2, D9Ertd393e, CDC25A, cdc25a2, cdc25a, MGC115549
Binding Site
Alternatives

pSer75
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN965831
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name cdc25A
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human cdc25A around the phosphorylation site of serine 75 (M-G-SP-S-E).
Specificity Cdc25A (phospho-Ser75) antibody detects endogenous levels of cdc25A only when phosphorylated at serine 75.

Application Details
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Application Notes WB: 1:500~1:1000.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell Cycle, Cancer, Kinases/Phosphatases
Restrictions For Research Use only

Publications
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Product Shimuta, Nakajo, Uto et al.: "Chk1 is activated transiently and targets Cdc25A for degradation at the Xenopus midblastula transition." in: The EMBO journal, Vol. 21, Issue 14, pp. 3694-703, 2002 (PubMed).

Jin, Shirogane, Xu et al.: "SCFbeta-TRCP links Chk1 signaling to degradation of the Cdc25A protein phosphatase." in: Genes & development, Vol. 17, Issue 24, pp. 3062-74, 2003 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A)", type "Antibodies"
Hosts Rabbit (135), Mouse (20)
Reactivities Human (150), Rat (Rattus) (61), Mouse (Murine) (55), Cow (Bovine) (44), Dog (Canine) (39), Chicken (29), Horse (Equine) (10), Sheep (Ovine) (10), Pig (Porcine) (5), Xenopus laevis (1), Zebrafish (1)
Applications Western Blotting (WB) (103), Immunofluorescence (IF) (46), ELISA (45), Immunoprecipitation (IP) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (29), Immunohistochemistry (IHC) (13), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (7), Alexa Fluor 488 (7), Alexa Fluor 555 (7), Alexa Fluor 647 (7), PE,Cy5.5 (7), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer76 (11), pSer75 (9), pSer124 (8), pSer177 (7), pThr506 (7), pSer292 (6), N-Term (5), pSer178 (5), Internal Region (4), pSer79 (3), Ser76 (2), Ser78 (2), pSer278 (2), AA 175-225 (1), Center (1), Exon 7 (1), Exon 9 (1), Ser75 (1), Ser75,pSer75 (1), pSer17 (1), pSer18 (1), pSer196 (1), pSer279 (1), pSer293 (1), pSer33 (1), pSer82 (1), pThr507 (1), pTyr15 (1)