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Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) antibody

Details for Product No. ABIN965835, Supplier: Log in to see
Antigen
  • CDC27
  • NV10210
  • ANAPC3
  • APC3
  • CDC27Hs
  • D0S1430E
  • D17S978E
  • HNUC
  • NUC2
  • AI452358
  • BC023187
Reactivity
Human
107
42
38
7
4
4
3
1
Host
Mouse
86
21
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
80
42
32
24
18
11
8
7
3
1
Supplier
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Immunogen Purified recombinant fragment of human CDC27 expressed in E. Coli.
Clone 5C12
Isotype IgG1
Purification Ascitic fluid
Alternative Name CDC27 (CDC27 Antibody Abstract)
Background Cdc27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. It is a component of the Anaphase Promoting Complex (APC), which is composed of eight protein subunits and is highly conserved in eucaryotic cells. The APC catalyzes the formation of the cyclin B ubiquitin conjugate that is responsible for the ubiquitin mediated proteolysis of B type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.
Synonyms: APC3, HNUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E,
Molecular Weight 91kDa
Gene ID 996
Research Area CD Antigens, Surface Receptors of Immune Cells, Cell Cycle, Coagulation
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Goto, Eddy: "Speriolin is a novel spermatogenic cell-specific centrosomal protein associated with the seventh WD motif of Cdc20." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 42128-38, 2004 (PubMed).

Background publications Koloteva-Levine, Pinchasi, Pereman et al.: "The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation." in: Molecular and cellular biology, Vol. 24, Issue 9, pp. 3577-87, 2004 (PubMed).