E-cadherin antibody

Catalog No. ABIN965839

Product Details anti-E-cadherin Antibody

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CDH1 expressed in E. Coli.

Clone: 7A2

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-E-cadherin antibody (ABIN965839)

Schietke, Warnecke, Wacker, Schödel, Mole, Campean, Amann, Goppelt-Struebe, Behrens, Eckardt, Wiesener: "The lysyl oxidases LOX and LOXL2 are necessary and sufficient to repress E-cadherin in hypoxia: insights into cellular transformation processes mediated by HIF-1." in: The Journal of biological chemistry, Vol. 285, Issue 9, pp. 6658-69, (2010) (PubMed).

Barrett, Lee, Lees, Prescott, Anderson, Phillips, Wesley, Parnell, Zhang, Drummond, Nimmo, Massey, Blaszczyk, Elliott, Cotterill, Dallal, Lobo, Mowat, Sanderson, Jewell, Newman, Edwards, Ahmad et al.: "Genome-wide association study of ulcerative colitis identifies three new susceptibility loci, including the HNF4A region. ..." in: Nature genetics, Vol. 41, Issue 12, pp. 1330-4, (2009) (PubMed).

Rigby: "Statistical reviewing for disability and rehabilitation." in: Disability and rehabilitation, Vol. 31, Issue 7, pp. 515-21, (2009) (PubMed).

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: CDH1 (CDH1 Products)

Synonyms: Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody

Background: E-Cadherin is a 120 kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There, it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calciumdependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-cell adhesion. These E-cadherin/catenin complexes associate with corical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression.
Tissue specificity: Non-neural epithelial tissues.
Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, CDH1

Molecular Weight: 135kDa

Gene ID: 999

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation