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Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Binding Site
Alternatives

Middle Region
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
2 references available
Catalog no. ABIN965881
Quantity 0.1mg
Price 288.75 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CHEK1(Serine/threonine-protein kinase Chk1)
Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to Middle residues of human CHEK1 (Serine/threonine-protein kinase Chk1)
Description The CHEK1 (Serine/threonine-protein kinase Chk1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. It binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25C creates a binding site for 14-3-3 protein which inhibits CDC25C. This prevents activation of the CDC2cyclin B complex and prevents mitotic entry. The CHEK1 phosphorylates CDC25C on serine-216.

Application Details
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Application Notes ELISA, Western blotting: 1µg/ml for 2hrs.
Purification Purified by antigen-specific affinity chromatography.
Buffer This antibody is stored in PBS, 50% glycerol
Preservative 0.01% Sodium Azide
Storage Store at -20°C.
Restrictions For Research Use only

Publications
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Product Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Flaggs, Plug, Dunks et al.: "Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes." in: Current biology : CB, Vol. 7, Issue 12, pp. 977-86, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (225), Mouse (20), Goat (6), Sheep (4)
Reactivities Human (243), Rat (Rattus) (131), Mouse (Murine) (124), Horse (Equine) (36), Cow (Bovine) (22), Chicken (21), Dog (Canine) (20), Pig (Porcine) (19), Monkey (4), Xenopus laevis (2), Yeast (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (Saccharomyces cerevisiae) (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (150), ELISA (93), Immunofluorescence (IF) (91), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunoprecipitation (IP) (18), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunocytochemistry (ICC) (10), Immunoelectron Microscopy (IEM) (5), Dot Blot (DB) (2), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Biotin (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), FITC (5), Gold (5), HRP (5), PE (5), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer317 (37), pSer280 (34), pSer345 (30), Ser296 (18), Internal Region (7), C-Term (6), pSer296 (6), Ser280 (4), AA 312-327 (3), N-Term (3), pSer296,Internal Region (3), Center (2), AA 1-474 (1), AA 250-300 (1), AA 259-311 (1), Internal Region,AA 251-279 (1), pSer286 (1), pSer301 (1)