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CHEK1 antibody (Checkpoint Kinase 1) (Middle Region)

Details for Product anti-CHEK1 Antibody No. ABIN965881, Supplier: Log in to see
Antigen
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
Alternatives
anti-Human CHEK1 antibody for Immunoprecipitation
Epitope
Middle Region
56
49
37
32
28
20
14
13
13
10
9
6
4
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
435
139
130
4
4
3
3
2
2
2
1
1
Host
Rabbit
381
47
21
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
12
12
11
8
8
8
5
3
3
3
3
3
3
3
3
Application
ELISA, Western Blotting (WB)
356
172
123
55
52
36
22
21
18
8
5
4
1
1
Supplier
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to Middle residues of human CHEK1 (Serine/threonine-protein kinase Chk1)
Purification Purified by antigen-specific affinity chromatography.
Alternative Name CHEK1(Serine/threonine-protein kinase Chk1) (CHEK1 Antibody Abstract)
Background The CHEK1 (Serine/threonine-protein kinase Chk1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. It binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25C creates a binding site for 14-3-3 protein which inhibits CDC25C. This prevents activation of the CDC2cyclin B complex and prevents mitotic entry. The CHEK1 phosphorylates CDC25C on serine-216.
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes ELISA, Western blotting: 1µg/ml for 2hrs.
Restrictions For Research Use only
Format Liquid
Buffer This antibody is stored in PBS, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Flaggs, Plug, Dunks et al.: "Atm-dependent interactions of a mammalian chk1 homolog with meiotic chromosomes." in: Current biology : CB, Vol. 7, Issue 12, pp. 977-86, 1998 (PubMed).

Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).