CHEK2 antibody (Checkpoint Kinase 2) (AA 481-531)

Details for Product anti-CHEK2 Antibody No. ABIN965887, Supplier: Log in to see
Antigen
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • LOC396525
Alternatives
anti-Human CHEK2 antibody for Enzyme Immunoassay
Epitope
AA 481-531
52
51
39
36
19
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12
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8
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3
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1
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1
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1
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1
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Reactivity
Human
487
219
199
19
19
16
13
8
5
4
2
1
1
1
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1
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Host
Mouse
353
135
8
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CHEK2 antibody is un-conjugated
10
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5
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2
2
2
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Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
399
125
123
107
89
66
48
43
27
6
6
5
2
1
1
Options
Supplier
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Immunogen Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.
Clone 1C12B8
Isotype IgG2b
Purification Ascitic fluid
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with LiFraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Synonyms: CDS1, LFS2, CHEK2
Molecular Weight 61kDa
Gene ID 11200
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Cybulski, Masojc, Oszutowska, Jaworowska, Grodzki, Waloszczyk, Serwatowski, Pankowski, Huzarski, Byrski, Górski, Jakubowska, Debniak, Wokolorczyk, Gronwald, Tarnowska, Serrano-Fernández, Lubinski et al.: "Constitutional CHEK2 mutations are associated with a decreased risk of lung and laryngeal cancers. ..." in: Carcinogenesis, Vol. 29, Issue 4, pp. 762-5, 2008 (PubMed).

Bell, Kim, Godwin, Schiripo, Harris, Haserlat, Wahrer, Haiman, Daly, Niendorf, Smith, Sgroi, Garber, Olopade, Le Marchand, Henderson, Altshuler, Haber, Freedman: "Genetic and functional analysis of CHEK2 (CHK2) variants in multiethnic cohorts." in: International journal of cancer. Journal international du cancer, Vol. 121, Issue 12, pp. 2661-7, 2007 (PubMed).

Product cited in: Nat, Nilbratt, Narkilahti, Winblad, Hovatta, Nordberg: "Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures." in: Glia, Vol. 55, Issue 4, pp. 385-99, 2007 (PubMed).

Antoni, Sodha, Collins, Garrett: "CHK2 kinase: cancer susceptibility and cancer therapy - two sides of the same coin?" in: Nature reviews. Cancer, Vol. 7, Issue 12, pp. 925-36, 2007 (PubMed).

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