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Checkpoint Kinase 2 (CHEK2) (pSer516) antibody

Details for Product No. ABIN965889, Supplier: Log in to see
Antigen
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Alternatives
anti-Human Checkpoint Kinase 2 antibody for Enzyme Immunoassay
Epitope
pSer516
43
41
39
32
24
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
408
161
160
10
8
7
7
7
5
1
1
1
Host
Rabbit
323
91
8
1
Conjugate
Un-conjugated
8
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Western Blotting (WB)
326
120
117
64
63
56
37
26
24
6
5
2
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).
Specificity Chk2 (phospho-Ser516) antibody detects endogenous levels of Chk2 only when phosphorylated at serine 516.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Chk2 (CHEK2 Antibody Abstract)
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes WB: 1:500~1:1000.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003

Lou, Minter-Dykhouse, Wu, Chen: "MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways." in: Nature, Vol. 421, Issue 6926, pp. 957-61, 2003

Product cited in: Schwarz, Lovly, Piwnica-Worms: "Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation." in: Molecular cancer research : MCR, Vol. 1, Issue 8, pp. 598-609, 2003