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Checkpoint Kinase 2 (CHEK2) (pSer516) antibody

Antigen

Checkpoint Kinase 2 (CHEK2)
Synonyms CDS1, CHK2, LFS2, RAD53, HuCds1, PP1425
Binding Site
Alternatives

pSer516
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN965889
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Chk2
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Chk2 around the phosphorylation site of serine 516 (Q-P-SP-T-S).
Specificity Chk2 (phospho-Ser516) antibody detects endogenous levels of Chk2 only when phosphorylated at serine 516.

Application Details
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Application Notes WB: 1:500~1:1000.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Restrictions For Research Use only

Publications
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Product Lou, Minter-Dykhouse, Wu et al.: "MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways." in: Nature, Vol. 421, Issue 6926, pp. 957-61, 2003 (PubMed).

Schwarz, Lovly, Piwnica-Worms: "Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation." in: Molecular cancer research : MCR, Vol. 1, Issue 8, pp. 598-609, 2003 (PubMed).

Wu, Chen: "Autophosphorylation of checkpoint kinase 2 at serine 516 is required for radiation-induced apoptosis." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36163-8, 2003 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Checkpoint Kinase 2 (CHEK2)", type "Antibodies"
Hosts Rabbit (223), Mouse (44)
Reactivities Human (259), Rat (Rattus) (119), Mouse (Murine) (112), Dog (Canine) (80), Cow (Bovine) (56), Horse (Equine) (54), Pig (Porcine) (54), Chicken (19), Monkey (4), Cat (Feline) (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (155), Immunofluorescence (IF) (113), ELISA (72), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), Immunohistochemistry (IHC) (48), Immunoprecipitation (IP) (26), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (18), Flow Cytometry (FACS) (9), Immunocytochemistry (ICC) (9), Immunoelectron Microscopy (IEM) (6)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Biotin (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), FITC (6), Gold (6), HRP (6), PE (6), PE,Cy3 (6), PE,Cy5 (6), PE,Cy5.5 (6), PE,Cy7 (6)
Epitopes pThr68 (34), N-Term (32), pSer516 (30), pThr387 (24), pSer33 (20), pSer19 (19), C-Term (13), Transcript Variant 1 (7), AA 2-18 (3), AA 481-531 (2), AA 514-518 (2), Internal Region (2), pSer33, pSer35 (2), AA 427-441 (1), AA 64-73,pThr68 (1), C-Term,AA 474-502 (1), N-Term,AA 120-150 (1), Ser33 (1), Ser516 (1), pSer379 (1), pThr383 (1)