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Cofilin 1 (Non-Muscle) (CFL1) (pSer3) antibody

Details for Product No. ABIN965910, Supplier: Log in to see
Antigen
  • 12/8
  • AA959946
  • ADF
  • Adf
  • ADF/cofilin
  • Cadf
  • cb86
  • CFL
  • cfl
  • CFL1
  • cfl1
  • CG4254
  • Cof
  • cofilin
  • cofilin/actin depolymerizing factor homolog
  • cofilin/ADF
  • D-cof
  • D61
  • Dmel\\CG4254
  • l(2)03101
  • l(2)k03101
  • l(2)k05633
  • l(2)k13409
  • ntf
  • sb:cb86
  • T8
  • Tsr
  • wu:fb18a04
  • wu:fk77b03
  • xac1
  • xac2
  • zgc:56501
Epitope
pSer3
55
31
29
21
19
15
14
13
13
11
9
7
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
307
144
130
9
8
4
4
3
2
1
1
1
1
Host
Rabbit
243
60
4
Conjugate
Un-conjugated
12
11
11
8
7
7
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
220
134
99
56
51
35
20
18
13
4
4
2
2
2
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V). 75 (M-G-SP-S-E).
Specificity Cofilin (phospho-Ser3) phosphorylated at serine 3.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Cofilin (CFL1 Antibody Abstract)
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Kobayashi, Nishita, Mishima, Ohashi, Mizuno: "MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration." in: The EMBO journal, Vol. 25, Issue 4, pp. 713-26, 2006 (PubMed).

Background publications Wang, Shibasaki, Mizuno: "Calcium signal-induced cofilin dephosphorylation is mediated by Slingshot via calcineurin." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12683-9, 2005 (PubMed).