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Cofilin 1 (Non-Muscle) (CFL1) (pSer3) antibody

Antigen

Cofilin 1 (Non-Muscle) (CFL1)
Synonyms
CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor ... show more
Binding Site
Alternatives

pSer3
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN965910
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Cofilin
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V). 75 (M-G-SP-S-E).
Specificity Cofilin (phospho-Ser3) phosphorylated at serine 3.
Synonyms CFL, xac1, MGC54000, cfl, MGC76274, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, CFL1, 12/8, ADF/cofilin, Adf, Cadf, D-cof, D61, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, DmelCG4254, CG4254, COFILIN

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Cytoskeleton, Microfilaments
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Wang, Shibasaki, Mizuno: "Calcium signal-induced cofilin dephosphorylation is mediated by Slingshot via calcineurin." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12683-9, 2005 (PubMed).

Kobayashi, Nishita, Mishima et al.: "MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration." in: The EMBO journal, Vol. 25, Issue 4, pp. 713-26, 2006 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Cofilin 1 (Non-Muscle) (CFL1)", type "Antibodies"
Hosts Rabbit (49), Mouse (9), Sheep (2)
Reactivities Human (55), Rat (Rattus) (25), Mouse (Murine) (22), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications Western Blotting (WB) (46), Immunohistochemistry (IHC) (35), ELISA (23), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunofluorescence (IF) (8), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoprecipitation (IP) (1)
Epitopes pSer3 (12), Ser3 (4), pTyr139 (4), pTyr140, pSer156 (4), Internal Region (3), N-Term (2), AA 1-166 (1), Center (1), Tyr88 (1), pTyr140 (1), pTyr88 (1)