Cofilin 1 (Non-Muscle) (CFL1) (pSer3) antibody

Details for Product No. ABIN965910
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Antigen
Synonyms
cfl1, xac1, xac2, cfl, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, 12/8, ADF, ADF/cofilin, Adf, CG4254, Cadf, D-cof, D61, Dmel\\CG4254, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizin ... show more
cfl1, xac1, xac2, cfl, cb86, sb:cb86, zgc:56501, wu:fb18a04, wu:fk77b03, 12/8, ADF, ADF/cofilin, Adf, CG4254, Cadf, D-cof, D61, Dmel\\CG4254, T8, Tsr, cofilin, cofilin/ADF, cofilin/actin depolymerizing factor homolog, l(2)03101, l(2)k03101, l(2)k05633, l(2)k13409, ntf, CFL, AA959946, Cof, CFL1 show less
Epitope
pSer3
(58), (30), (22), (20), (18), (15), (14), (13), (12), (12), (6), (6), (6), (5), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(302), (149), (139), (2), (2), (2)
Host
Rabbit
(261), (42), (3)
Conjugate
Un-conjugated
(11), (11), (11), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5)
Application
Immunohistochemistry (IHC)
(202), (109), (94), (52), (50), (35), (19), (17), (9), (8), (3), (2), (2), (1)
Pubmed 2 references available
Quantity 100 μg
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human cofilin around the phosphorylation site of serine 3 (M-A-SP-G-V). 75 (M-G-SP-S-E).
Specificity Cofilin (phospho-Ser3) phosphorylated at serine 3.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Cofilin (CFL1 Antibody Abstract)
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Kobayashi, Nishita, Mishima et al.: "MAPKAPK-2-mediated LIM-kinase activation is critical for VEGF-induced actin remodeling and cell migration." in: The EMBO journal, Vol. 25, Issue 4, pp. 713-26, 2006 (PubMed).

Background publications Wang, Shibasaki, Mizuno: "Calcium signal-induced cofilin dephosphorylation is mediated by Slingshot via calcineurin." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12683-9, 2005 (PubMed).

Validation Images
Catalog No. ABIN965910
398.75 $
Plus shipping costs $45.00
Quantity
Price
100 μg
398.75 $

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