Keratin 10/13 antibody

Catalog No. ABIN965954

Product Details

Target: Keratin 10/13

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Concentrated.

Immunogen: Cytoskeleton preparation from HeLa Cells

Clone: B28-1 (DE-K13)

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 with Auto ProbeIII. The optimal conditions should be determined by the individual laboratory. Enzymatic predigestion is not recommended.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Squamous Granulocytes Carcinomas, Esophagus, or Tongue

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10+13 (56.5 & 53kDa)

Background: This antibody reacts with Cytokeratin 10 and 13 (56.5 and 53 kD) polypeptide. On formalin-fixed,Paraffinembedded tissue sections, this antibody recognizes only one of the two cytokeratins: Cytokeratin 13, that is found in non-squamous stratified epithelia.