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EGF antibody (Epidermal Growth Factor)

Details for Product anti-EGF Antibody No. ABIN966030, Supplier: Log in to see
Antigen
  • AI790464
  • CEGF
  • HOMG4
  • URG
Alternatives
anti-Human EGF antibody for ELISA (Capture)
Reactivity
Human
290
105
59
15
12
7
4
3
2
1
Host
Mouse
263
136
29
10
7
3
Clonality (Clone)
Monoclonal ()
Conjugate
This EGF antibody is un-conjugated
47
24
21
9
8
8
5
5
4
4
4
4
4
4
4
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
282
270
70
48
46
41
27
22
20
17
14
14
11
8
6
5
4
2
2
2
2
1
Supplier
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Immunogen Purified recombinant fragment of human EGF expressed in E. Coli.
Clone 9D7F11
Isotype IgG1
Purification Ascitic fluid
Alternative Name EGF (EGF Antibody Abstract)
Background Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
Synonyms: URG, HOMG4, EGF
Molecular Weight 134kDa
Gene ID 1950
Research Area Cancer, Growth Factors
Pathways NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Yacoub, McKinstry, Hinman, Chung, Dent, Hagan: "Epidermal growth factor and ionizing radiation up-regulate the DNA repair genes XRCC1 and ERCC1 in DU145 and LNCaP prostate carcinoma through MAPK signaling." in: Radiation research, Vol. 159, Issue 4, pp. 439-52, 2003

Background publications Soubeyran, Kowanetz, Szymkiewicz, Langdon, Dikic: "Cbl-CIN85-endophilin complex mediates ligand-induced downregulation of EGF receptors." in: Nature, Vol. 416, Issue 6877, pp. 183-7, 2002

Gual, Giordano, Williams, Rocchi, Van Obberghen, Comoglio: "Sustained recruitment of phospholipase C-gamma to Gab1 is required for HGF-induced branching tubulogenesis." in: Oncogene, Vol. 19, Issue 12, pp. 1509-18, 2000

Gout, Dhand, Panayotou, Fry, Hiles, Otsu, Waterfield: "Expression and characterization of the p85 subunit of the phosphatidylinositol 3-kinase complex and a related p85 beta protein by using the baculovirus expression system." in: The Biochemical journal, Vol. 288 ( Pt 2), pp. 395-405, 1993