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Epidermal Growth Factor Receptor (EGFR) (pSer1070) antibody

Antigen

Epidermal Growth Factor Receptor (EGFR)
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33,  ... show more
Binding Site
Alternatives

pSer1070
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966032
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name EGFR
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human EGFR around the phosphorylation site of serine 1070 (R-Y-SP-S-D).
Specificity EGFR (phospho-Ser1070) antibody detects endogenous levels of EGFR only when phosphorylated at serine1070.
Synonyms ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Tyrosine Kinases, Growth Factors, Hormones, Receptors
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Heisermann, Gill: "Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo." in: The Journal of biological chemistry, Vol. 263, Issue 26, pp. 13152-8, 1988 (PubMed).

Gamou, Shimizu: "Calphostin-C stimulates epidermal growth factor receptor phosphorylation and internalization via light-dependent mechanism." in: Journal of cellular physiology, Vol. 158, Issue 1, pp. 151-9, 1994 (PubMed).

Gamou, Shimizu: "Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor." in: FEBS letters, Vol. 357, Issue 2, pp. 161-4, 1995 (PubMed).

Feinmesser, Wicks, Taverner et al.: "Ca2+/calmodulin-dependent kinase II phosphorylates the epidermal growth factor receptor on multiple sites in the cytoplasmic tail and serine 744 within the kinase domain to regulate signal generation." in: The Journal of biological chemistry, Vol. 274, Issue 23, pp. 16168-73, 1999 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts Rabbit (576), Mouse (195), Rat (18), Sheep (9), Goat (3), Mouse (Murine) (1)
Reactivities Human (783), Mouse (Murine) (350), Rat (Rattus) (334), Pig (Porcine) (88), Dog (Canine) (80), Cow (Bovine) (59), Horse (Equine) (52), Chicken (28), Sheep (Ovine) (23), Rabbit (18), Cat (Feline) (1)
Applications Western Blotting (WB) (458), ELISA (261), Immunofluorescence (IF) (218), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (164), Immunoprecipitation (IP) (151), Immunohistochemistry (IHC) (126), Flow Cytometry (FACS) (91), Immunocytochemistry (ICC) (55), Immunohistochemistry (Frozen Sections) (IHC (fro)) (29), Blocking Antibody (Inhibition) (17), Dot Blot (Dot) (16), Immunohistochemistry (Fixed) (IHC (fx)) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (11), Immunoelectron Microscopy (IEM) (4), Functional Studies (Func) (3), Cellular Assay (CA) (2), Enzyme Immunoassay (EIA) (2), Immunoassay (IA) (2), Immunostaining (ISt) (2), Blocking Antibody (Inhibition) (1), Cell-ELISA (cELISA) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Neutralization (Neut) (1)
Conjugates Biotin (39), Alexa Fluor 488 (25), Alexa Fluor 350 (24), Alexa Fluor 555 (24), Alexa Fluor 647 (24), PE,Cy5.5 (23), FITC (9), PE (5), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), Gold (3), HRP (3), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RPE (2), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes C-Term (20), Extracellular Domain (18), pTyr1092 (17), pTyr1197 (17), pTyr869 (17), pTyr1110 (16), pSer1070 (15), pThr678 (15), pThr693 (14), pTyr1172 (12), pTyr1173 (9), Internal Region (8), pSer1026 (8), pSer695 (8), pTyr1016 (8), pTyr1068 (8), pTyr845 (8), pSer1166 (7), pSer1190 (7), pSer768 (7), pTyr1045 (7), pTyr1125 (7), pTyr998 (6), pSer991 (5), pTyr1138 (5), Ser1070 (4), Tyr1092 (4), Tyr1110 (4), Tyr1172 (4), Tyr1197 (4), Tyr869 (4), pSer1142 (4), AA 1165-1186 (3), AA 1189-1199 (3), N-Term (3), Thr693 (3), Tyr1016 (3), pTyr1069 (3), pTyr992 (3), AA 985-996 (2), Extracellular, Ligand Binding Domain (2), Extracellular, non Ligand Binding Domain (2), Ser1071 (2), Ser695 (2), Ser991 (2), Thr678 (2), Tyr1068 (2), Tyr1125 (2), Tyr978 (2), Tyr998 (2), pSer1047 (2), pThr654 (2), AA 1165-1185 (1), AA 1166-1180 (1), AA 1189-1199,pTyr1197 (1), AA 424-605 (1), C-Term, Ser1026 (1), C-Term,Ser1081 (1), Extracellular,Non-Ligand binding Domain (1), Ligand binding Domain (1), Middle Region (1), Ser1026 (1), pSer1045 (1), pSer1070, pSer1071 (1), pSer1071 (1), pSer332 (1), pThr637 (1), pTyr1086 (1), pTyr142 (1), pTyr537 (1)