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ELK1, Member of ETS Oncogene Family (ELK1) (pSer383) antibody

Antigen

ELK1, Member of ETS Oncogene Family (ELK1)

Synonyms Elk-1
Binding Site
Alternatives

pSer383

Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
6 references available
Catalog no. ABIN966058
Quantity 100ug  (100ug/100ul)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name Elk-1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Elk-1 around the phosphorylation site of serine 383 (T-L-SP-P-I).
Specificity Elk-1 (phospho-Ser383) antibody detects endogenous levels of Elk-1 only when phosphorylated at serine 383.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Kortenjann, Thomae, Shaw: "Inhibition of v-raf-dependent c-fos expression and transformation by a kinase-defective mutant of the mitogen-activated protein kinase Erk2." in: Molecular and cellular biology, Vol. 14, Issue 7, pp. 4815-24, 1994 (PubMed).

Janknecht, Ernst, Pingoud et al.: "Activation of ternary complex factor Elk-1 by MAP kinases." in: The EMBO journal, Vol. 12, Issue 13, pp. 5097-104, 1994 (PubMed).

Marais, Wynne, Treisman: "The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain." in: Cell, Vol. 73, Issue 2, pp. 381-93, 1993 (PubMed).

Xing, Ginty, Greenberg: "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase." in: Science (New York, N.Y.), Vol. 273, Issue 5277, pp. 959-63, 1996 (PubMed).

Cavigelli, Dolfi, Claret et al.: "Induction of c-fos expression through JNK-mediated TCF/Elk-1 phosphorylation." in: The EMBO journal, Vol. 14, Issue 23, pp. 5957-64, 1996 (PubMed).

Conner: "Is there a secular answer to euthanasia, American style?" in: Angelicum, Vol. 73, pp. 381-400, 1999 (PubMed).

Alternatives

Alternatives for antigen "ELK1, Member of ETS Oncogene Family (ELK1)", type "Antibodies"
Hosts Rabbit (138), Mouse (21)
Reactivities Human (156), Rat (Rattus) (82), Mouse (Murine) (77), Cow (Bovine) (34), Horse (Equine) (23), Dog (Canine) (20), Pig (Porcine) (15), Guinea Pig (10), Sheep (Ovine) (5), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (78), ELISA (66), Immunohistochemistry (IHC) (52), Immunofluorescence (IF) (42), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Dot Blot (Dot) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer383 (30), pSer389 (19), pThr417 (17), C-Term (4), Ser383 (4), pS383 (4), N-Term (3), Ser389 (2), Thr417 (2), AA 379-392 (1), Internal Region (1), Phosphospecific (1), pSer100 (1), pSer424 (1), phospho (1)