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Exonuclease 1 antibody (EXO1) (N-Term)

Details for Product anti-EXO1 Antibody No. ABIN966108, Supplier: Log in to see
Antigen
  • 5730442G03Rik
  • ATWEX
  • ATWRNEXO
  • EXO1
  • exoi
  • EXONUCLEASE
  • F15D2.37
  • F15D2_37
  • F18A5.260
  • F18A5_260
  • fc16e04
  • HEX1
  • hExoI
  • Msa
  • Werner syndrome-like exonuclease
  • WEX
  • wu:fc16e04
  • zgc:55521
Alternatives
anti-Human Exonuclease 1 antibody for Western Blotting
Epitope
N-Term
13
12
12
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
90
31
14
1
Host
Rabbit
55
23
20
Clonality
Polyclonal
Conjugate
This Exonuclease 1 antibody is un-conjugated
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
73
38
19
11
7
5
4
3
2
1
1
Supplier
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Immunogen Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to N-terminal residues of human exonuclease 1
Purification Purified by antigen-specific affinity chromatography.
Alternative Name EXO1 (EXO1 Antibody Abstract)
Background EXO1 (exonuclease 1) is a 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatchcontaining DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5' overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5' end of a downstream Okazaki fragment. EXO1 is required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. It is essential for male and female meiosis. EXO1 interacts with the MLH1-PMS2 heterodimer via MLH1. It interacts with MSH3 and with the MSH2-MSH6 heterodimer via MSH2, and this interaction may increase the processivity of the 5'->3' exonuclease activity. EXO1 interacts with PCNA, and this interaction may both stimulate the cryptic 3'->5' exonuclease activity and suppress the 5'->3' exonuclease activity. EXO1 interacts with WRN, and this interaction stimulates both the 5'->3' exonuclease activity and cleavage of 5' overhanging flap structures. It also interacts with RECQL/RECQ1, and this interaction stimulates cleavage of 5' overhanging flap structures.
Research Area Metabolism
Pathways DNA Damage Repair
Application Notes ELISA, Western blotting: 1µg/ml for 2hrs.
Restrictions For Research Use only
Format Liquid
Buffer This antibody is stored in PBS, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Genschel, Modrich: "Functions of MutLalpha, replication protein A (RPA), and HMGB1 in 5'-directed mismatch repair." in: The Journal of biological chemistry, Vol. 284, Issue 32, pp. 21536-44, 2009 (PubMed).

Sharma, Sommers, Driscoll, Uzdilla, Wilson, Brosh: "The exonucleolytic and endonucleolytic cleavage activities of human exonuclease 1 are stimulated by an interaction with the carboxyl-terminal region of the Werner syndrome protein." in: The Journal of biological chemistry, Vol. 278, Issue 26, pp. 23487-96, 2003 (PubMed).

Background publications Zhang, Yuan, Presnell, Tian, Gao, Tomkinson, Gu, Li: "Reconstitution of 5'-directed human mismatch repair in a purified system." in: Cell, Vol. 122, Issue 5, pp. 693-705, 2005 (PubMed).

Dzantiev, Constantin, Genschel, Iyer, Burgers, Modrich: "A defined human system that supports bidirectional mismatch-provoked excision." in: Molecular cell, Vol. 15, Issue 1, pp. 31-41, 2004 (PubMed).

Nielsen, Jäger, Lützen, Bundgaard, Rasmussen: "Characterization of human exonuclease 1 in complex with mismatch repair proteins, subcellular localization and association with PCNA." in: Oncogene, Vol. 23, Issue 7, pp. 1457-68, 2004 (PubMed).

Alam, Gorman, Jaeger, Kelsell, Leigh, Ratnavel, Murdoch, Houlston, Aaltonen, Roylance, Tomlinson: "Germline deletions of EXO1 do not cause colorectal tumors and lesions which are null for EXO1 do not have microsatellite instability." in: Cancer genetics and cytogenetics, Vol. 147, Issue 2, pp. 121-7, 2003 (PubMed).

Sun, Zheng, Shen: "Functional alterations of human exonuclease 1 mutants identified in atypical hereditary nonpolyposis colorectal cancer syndrome." in: Cancer research, Vol. 62, Issue 21, pp. 6026-30, 2002 (PubMed).

Genschel, Bazemore, Modrich: "Human exonuclease I is required for 5' and 3' mismatch repair." in: The Journal of biological chemistry, Vol. 277, Issue 15, pp. 13302-11, 2002 (PubMed).

Schmutte, Sadoff, Shim, Acharya, Fishel: "The interaction of DNA mismatch repair proteins with human exonuclease I." in: The Journal of biological chemistry, Vol. 276, Issue 35, pp. 33011-8, 2001 (PubMed).

Jäger, Rasmussen, Bisgaard, Singh, Nielsen, Rasmussen: "HNPCC mutations in the human DNA mismatch repair gene hMLH1 influence assembly of hMutLalpha and hMLH1-hEXO1 complexes." in: Oncogene, Vol. 20, Issue 27, pp. 3590-5, 2001 (PubMed).

Lee, Wilson: "The RAD2 domain of human exonuclease 1 exhibits 5' to 3' exonuclease and flap structure-specific endonuclease activities." in: The Journal of biological chemistry, Vol. 274, Issue 53, pp. 37763-9, 2000 (PubMed).

Rasmussen, Rasmussen, Lee, Rasmussen, Wilson, Nielsen, Bisgaard et al.: "Identification of factors interacting with hMSH2 in the fetal liver utilizing the yeast two-hybrid system. In vivo interaction through the C-terminal domains of hEXO1 and hMSH2 and comparative ..." in: Mutation research, Vol. 460, Issue 1, pp. 41-52, 2000 (PubMed).

Qiu, Qian, Chen, Guan, Shen: "Human exonuclease 1 functionally complements its yeast homologues in DNA recombination, RNA primer removal, and mutation avoidance." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17893-900, 1999 (PubMed).

Schmutte, Marinescu, Sadoff, Guerrette, Overhauser, Fishel: "Human exonuclease I interacts with the mismatch repair protein hMSH2." in: Cancer research, Vol. 58, Issue 20, pp. 4537-42, 1998 (PubMed).

Tishkoff, Amin, Viars, Arden, Kolodner: "Identification of a human gene encoding a homologue of Saccharomyces cerevisiae EXO1, an exonuclease implicated in mismatch repair and recombination." in: Cancer research, Vol. 58, Issue 22, pp. 5027-31, 1998 (PubMed).