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Ezrin (EZR) (pThr566) antibody

Details for Product No. ABIN966109, Supplier: Log in to see
Antigen
  • ezr
  • vil2
  • wu:fc05c03
  • wu:fj31d06
  • cvil
  • cvl
  • CVIL
  • CVL
  • VIL2
  • AW146364
  • R75297
  • Vil2
  • p81
  • ATVLN2
  • T11A7.16
  • T11A7_16
  • villin 2
Epitope
pThr566
29
28
23
23
22
21
14
13
11
8
6
5
5
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
317
177
155
13
10
8
6
5
4
3
3
2
2
Host
Rabbit
260
31
25
2
Conjugate
Un-conjugated
11
9
8
5
5
5
4
4
4
4
4
4
2
1
1
Application
Immunohistochemistry (IHC)
250
104
60
45
41
23
6
6
5
3
3
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 566 (Y-K-TP-L-R).
Specificity Ezrin (phospho-Tyr566) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 566.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Ezrin (EZR Antibody Abstract)
Research Area Cancer, Cytoskeleton, Microfilaments
Application Notes WB: 1:500~1:1000.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Shiue, Musch, Wang et al.: "Akt2 phosphorylates ezrin to trigger NHE3 translocation and activation." in: The Journal of biological chemistry, Vol. 280, Issue 2, pp. 1688-95, 2005 (PubMed).