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Ezrin (EZR) (pTyr353) antibody

Antigen

Ezrin (EZR)

Synonyms CVL, CVIL, VIL2, MGC1584, FLJ26216, DKFZp762H157, EZR, vil2, wu:fc05c03, wu:fj31d06, cvl, cvil, MGC97499
Binding Site
Alternatives

pTyr353

Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966110
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name Ezrin
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Ezrin around the phosphorylation site of tyrosine 353 (Q-D-YP-E-E).
Specificity Ezrin (phospho-Tyr353) antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353.

Application Details

Application Notes WB: 1:500~1:1000.
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Cancer, Cytoskeleton, Microfilaments
Restrictions For Research Use only

Publications

Product Gautreau, Poullet, Louvard et al.: "Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 13, pp. 7300-5, 1999 (PubMed).

Wang, Pfeiffer, Gaarde: "Activation of SRC tyrosine kinases in response to ICAM-1 ligation in pulmonary microvascular endothelial cells." in: The Journal of biological chemistry, Vol. 278, Issue 48, pp. 47731-43, 2003 (PubMed).

Zhao, Shiue, Palkon et al.: "Ezrin regulates NHE3 translocation and activation after Na+-glucose cotransport." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 25, pp. 9485-90, 2004 (PubMed).

Alternatives

Alternatives for antigen "Ezrin (EZR)", type "Antibodies"
Hosts Rabbit (103), Mouse (17), Goat (8)
Reactivities Human (127), Mouse (Murine) (76), Rat (Rattus) (65), Dog (Canine) (31), Cow (Bovine) (30), Horse (Equine) (28), Pig (Porcine) (28), Guinea Pig (23), Chicken (5), Hamster (2), Kangaroo (2)
Applications Western Blotting (WB) (98), ELISA (32), Immunofluorescence (IF) (27), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26), Immunohistochemistry (IHC) (16), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Biotin (4), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr353 (13), pThr567 (8), pThr566 (6), Thr567 (5), C-Term (3), AA 362-585 (2), AA 479-498 (2), Internal Region (2), N-Term (1), Ser566 (1), Thr566 (1), Tyr566 (1), pThr353 (1), pThr567, pThr564, pThr558 (1), pTyr146 (1), pTyr40 (1), pTyr478 (1)