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Forkhead Box O1 (FOXO1) (pSer256) antibody

Antigen

Forkhead Box O1 (FOXO1)
Synonyms FKH1, FKHR, FOXO1A, Afxh, Fkhr1, Foxo1a, AI876417, Fkhr, FOXO1, fkh1, fkhr, foxo1a, xFoxO1, MGC79506
Binding Site
Alternatives

pSer256
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN966146
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name FKHR
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 256 (A-A-SP-M-D).
Specificity FKHR (phospho-Ser256) antibody detects endogenous levels of FKHR only when phosphorylated at serine 256.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Smith, Norton, Gorospe et al.: "Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity." in: The Journal of cell biology, Vol. 169, Issue 2, pp. 331-9, 2005 (PubMed).

Gan, Zheng, Chabot et al.: "Nuclear/cytoplasmic shuttling of the transcription factor FoxO1 is regulated by neurotrophic factors." in: Journal of neurochemistry, Vol. 93, Issue 5, pp. 1209-19, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Forkhead Box O1 (FOXO1)", type "Antibodies"
Hosts Rabbit (136), Mouse (21), Goat (6), Sheep (1)
Reactivities Human (163), Mouse (Murine) (96), Rat (Rattus) (91), Cow (Bovine) (52), Dog (Canine) (52), Pig (Porcine) (50), Chicken (29), Horse (Equine) (28), Rabbit (18), Monkey (2), Xenopus laevis (2), Zebrafish (2)
Applications Western Blotting (WB) (112), Immunofluorescence (IF) (64), Immunohistochemistry (IHC) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), ELISA (35), Immunocytochemistry (ICC) (16), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (4), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (3), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer319 (15), pSer256 (14), AA 242-655 (7), Internal Region (6), N-Term (5), C-Term (3), Ser256 (3), Ser319 (2), pSer329 (2), AA 250-300 (1), AA 353-655 (1), Center,Asp469 (1), pSer253 (1)