Forkhead Box O1 (FOXO1) (pSer256) antibody

Details for Product No. ABIN966146
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Antigen
Synonyms FOXO1, FOXO1A, fkh1, fkhr, foxo1a, xFoxO1, FKH1, FKHR, AI876417, Afxh, Fkhr1, Foxo1a, Fkhr, FoxO1A, FoxO1a.1, foxo1, zgc:153388
Epitope
pSer256
(40), (38), (25), (16), (14), (14), (13), (11), (10), (8), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(310), (191), (165), (77), (77), (77), (61), (2), (1), (1), (1)
Host
Rabbit
(256), (47), (8), (1)
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Immunohistochemistry (IHC)
(234), (112), (85), (72), (60), (54), (37), (4), (4), (3), (2)
Pubmed 2 references available
Quantity 100 µg
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Catalog No. ABIN966146
385.00 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 256 (A-A-SP-M-D).
Specificity FKHR (phospho-Ser256) antibody detects endogenous levels of FKHR only when phosphorylated at serine 256.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name FKHR
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Smith, Norton, Gorospe et al.: "Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity." in: The Journal of cell biology, Vol. 169, Issue 2, pp. 331-9, 2005 (PubMed).

Gan, Zheng, Chabot et al.: "Nuclear/cytoplasmic shuttling of the transcription factor FoxO1 is regulated by neurotrophic factors." in: Journal of neurochemistry, Vol. 93, Issue 5, pp. 1209-19, 2005 (PubMed).

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