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Forkhead Box O1 (FOXO1) (pSer319) antibody

Antigen

Forkhead Box O1 (FOXO1)
Synonyms FKH1, FKHR, FOXO1A, Afxh, Fkhr1, Foxo1a, AI876417, Fkhr, FOXO1, fkh1, fkhr, foxo1a, xFoxO1, MGC79506
Binding Site
Alternatives

pSer319
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN966147
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name FKHR
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).
Specificity FKHR (phospho-Ser319) antibody detects endogenous levels of FKHR only when phosphorylated at serine 319.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Rena, Woods, Prescott et al.: "Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion." in: The EMBO journal, Vol. 21, Issue 9, pp. 2263-71, 2002 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Forkhead Box O1 (FOXO1)", type "Antibodies"
Hosts Rabbit (135), Mouse (21), Goat (6), Sheep (1)
Reactivities Human (160), Mouse (Murine) (78), Rat (Rattus) (78), Cow (Bovine) (52), Dog (Canine) (50), Pig (Porcine) (50), Chicken (29), Horse (Equine) (28), Rabbit (18), Xenopus laevis (2), Zebrafish (2)
Applications Western Blotting (WB) (111), Immunofluorescence (IF) (63), Immunohistochemistry (IHC) (44), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (38), ELISA (35), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (4), Immunoprecipitation (IP) (4), Immunoelectron Microscopy (IEM) (2), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (3), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer256 (20), pSer319 (18), AA 242-655 (8), N-Term (7), Internal Region (6), C-Term (4), Ser256 (3), Ser319 (2), pSer329 (2), AA 250-300 (1), AA 254-258 (1), AA 353-655 (1), Center (1), Center,Asp469 (1), pSer253 (1)