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FOXO1 antibody (Forkhead Box O1) (pSer319)

Details for Product anti-FOXO1 Antibody No. ABIN966147, Supplier: Login to see
Antigen
  • FOXO1
  • FOXO1A
  • fkh1
  • fkhr
  • foxo1a
  • xFoxO1
  • FKH1
  • FKHR
  • AI876417
  • Afxh
  • Fkhr1
  • Foxo1a
  • Fkhr
  • FoxO1A
  • FoxO1a.1
  • foxo1
  • zgc:153388
Alternatives
anti-Human FOXO1 antibody for Immunohistochemistry
Epitope
pSer319
52
39
26
24
19
16
16
15
14
10
9
8
6
5
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
380
189
164
17
17
8
4
3
2
2
1
1
1
1
Host
Rabbit
302
62
21
2
Conjugate
This FOXO1 antibody is un-conjugated
7
7
6
5
4
4
4
4
4
4
4
4
1
1
1
Application
Immunohistochemistry (IHC)
298
143
115
82
67
47
35
9
7
5
4
3
3
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FKHR around the phosphorylation site of serine 319 (T-S-SP-N-A).
Specificity FKHR (phospho-Ser319) antibody detects endogenous levels of FKHR only when phosphorylated at serine 319.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name FKHR (FOXO1 Antibody Abstract)
Pathways PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Rena, Woods, Prescott et al.: "Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion." in: The EMBO journal, Vol. 21, Issue 9, pp. 2263-71, 2002 (PubMed).

Woods, Rena, Morrice et al.: "The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site." in: The Biochemical journal, Vol. 355, Issue Pt 3, pp. 597-607, 2001 (PubMed).

Rena, Prescott, Guo et al.: "Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting." in: The Biochemical journal, Vol. 354, Issue Pt 3, pp. 605-12, 2001 (PubMed).