FOXO3 antibody (Forkhead Box O3) (pSer253)

Details for Product anti-FOXO3 Antibody No. ABIN966150, Supplier: Log in to see
Antigen
  • FOXO3
  • FKHRL1
  • FOXO3A
  • zgc:92176
  • wu:fb50g02
  • wu:fi33f07
  • foxo3a
  • xfoxo3
  • foxO3
  • foxO
  • AF6q21
  • FKHRL1P2
  • FOXO2
  • 1110048B16Rik
  • 2010203A17Rik
  • C76856
  • Fkhr2
  • Foxo3a
  • FoxO3A
  • Fkhrl1
  • FOXO-3
  • forkhead box O3
  • forkhead box O3A
  • FOXO3
  • foxo3a
  • foxo3
  • foxO3
  • Foxo3
Alternatives
anti-Human FOXO3 antibody for Immunofluorescence (fixed cells)
Epitope
pSer253
49
47
37
34
14
14
14
10
8
8
5
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
299
144
116
22
17
11
11
9
8
7
6
5
4
4
3
2
1
1
1
Host
Rabbit
216
60
23
1
Conjugate
This FOXO3 antibody is un-conjugated
8
8
7
5
5
5
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC)
247
145
114
96
87
28
27
24
10
5
3
2
1
1
1
1
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).
Specificity FKHRL1 (phospho-Ser253) antibody detects endogenous levels of FKHRL1 only when phosphorylated at serine 253.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name FKHRL1 (FOXO3 Antibody Abstract)
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Smith, Norton, Gorospe, Jiang, Nemoto, Holbrook, Finkel, Kusiak: "Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity." in: The Journal of cell biology, Vol. 169, Issue 2, pp. 331-9, 2005 (PubMed).

Essafi, Fernández de Mattos, Hassen, Soeiro, Mufti, Thomas, Medema, Lam: "Direct transcriptional regulation of Bim by FoxO3a mediates STI571-induced apoptosis in Bcr-Abl-expressing cells." in: Oncogene, Vol. 24, Issue 14, pp. 2317-29, 2005 (PubMed).

Brunet, Park, Tran, Hu, Hemmings, Greenberg: "Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a)." in: Molecular and cellular biology, Vol. 21, Issue 3, pp. 952-65, 2001 (PubMed).

Goode, Grauer, Gums: "Infectious vaginitis. Selecting therapy and preventing recurrence." in: Postgraduate medicine, Vol. 96, Issue 6, pp. 85-8, 91-8, 1994 (PubMed).

Background publications Brunet, Bonni, Zigmond, Lin, Juo, Hu, Anderson, Arden, Blenis, Greenberg: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999 (PubMed).