Forkhead Box O3 (FOXO3) (pSer253) antibody

Antigen: Forkhead Box O3 (FOXO3)

Synonyms: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, Fkhr2, C76856, Foxo3a, 1110048B16Rik, 2010203A17Rik, Fkhrl1, FoxO3, FoxO3A, FOXO3, zgc:92176, wu:fb50g02, wu:fi33f07, foxo3, foxo3l, xFox06, foxO3, foxO

Binding Site: pSer253

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966150

Additional Information

Alternative name: FKHRL1

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human FKHRL1 around the phosphorylation site of serine 253 (A-V-SP-M-D).

Specificity: FKHRL1 (phospho-Ser253) antibody detects endogenous levels of FKHRL1 only when phosphorylated at serine 253.

Synonyms: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677, Fkhr2, C76856, Foxo3a, 1110048B16Rik, 2010203A17Rik, Fkhrl1, FoxO3, FoxO3A, FOXO3, zgc:92176, wu:fb50g02, wu:fi33f07, foxo3, foxo3l, xFox06, foxO3, foxO

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Goode, Grauer, Gums: "Infectious vaginitis. Selecting therapy and preventing recurrence." in: Postgraduate medicine, Vol. 96, Issue 6, pp. 85-8, 91-8, 1994 (PubMed).

Brunet, Bonni, Zigmond et al.: "Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor." in: Cell, Vol. 96, Issue 6, pp. 857-68, 1999 (PubMed).

Brunet, Park, Tran et al.: "Protein kinase SGK mediates survival signals by phosphorylating the forkhead transcription factor FKHRL1 (FOXO3a)." in: Molecular and cellular biology, Vol. 21, Issue 3, pp. 952-65, 2001 (PubMed).

Essafi, Fernández de Mattos, Hassen et al.: "Direct transcriptional regulation of Bim by FoxO3a mediates STI571-induced apoptosis in Bcr-Abl-expressing cells." in: Oncogene, Vol. 24, Issue 14, pp. 2317-29, 2005 (PubMed).

Smith, Norton, Gorospe et al.: "Phosphorylation of p66Shc and forkhead proteins mediates Abeta toxicity." in: The Journal of cell biology, Vol. 169, Issue 2, pp. 331-9, 2005 (PubMed).