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GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1) (pSer142) antibody

Antigen

GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1)
Synonyms GF1, GF-1, NFE1, XLTT, ERYF1, Gf-1, Gata-1, Eklf, Eryf1, GATA1, GATA transcription factor 1, gata-1, Gata1, si:dkeyp-77f7.5
Binding Site
Alternatives

pSer142
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966187
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name GATA1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human GATA-1 around the phosphorylation site of serine 142 (R-L-SP-P-D).
Specificity GATA-1 (phospho-Ser142) antibody detects endogenous levels of GATA-1 only when phosphorylated at serine 142.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Organogenesis, Hematopoietic Progenitors, Transcription Factors
Restrictions For Research Use only

Publications
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Product Trainor, Evans, Felsenfeld et al.: "Structure and evolution of a human erythroid transcription factor." in: Nature, Vol. 343, Issue 6253, pp. 92-6, 1990 (PubMed).

Zon, Tsai, Burgess et al.: "The major human erythroid DNA-binding protein (GF-1): primary sequence and localization of the gene to the X chromosome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 2, pp. 668-72, 1990 (PubMed).

Nichols, Crispino, Poncz et al.: "Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1." in: Nature genetics, Vol. 24, Issue 3, pp. 266-70, 2000 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1)", type "Antibodies"
Hosts Rabbit (97), Mouse (22), Goat (5), Rat (1)
Reactivities Human (121), Rat (Rattus) (80), Mouse (Murine) (74), Cow (Bovine) (25), Pig (Porcine) (23), Dog (Canine) (21), Horse (Equine) (19), Rabbit (4), Cat (Feline) (1), Chicken (1)
Applications Western Blotting (WB) (86), Immunofluorescence (IF) (60), ELISA (43), Immunohistochemistry (IHC) (31), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (3), Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer310 (16), pSer142 (14), Ser142 (3), Ser310 (3), Internal Region (2), C-Term (1), Center (1), N-Term (1), Pro401 (1), pSer21 (1)