GCK antibody

Catalog No. ABIN966192

Product Details anti-GCK Antibody

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCK antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human GCK expressed in E. Coli.

Clone: 4G6

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GCK antibody (ABIN966192)

Barbetti, Cobo-Vuilleumier, Dionisi-Vici, Toni, Ciampalini, Massa, Rodriguez-Bada, Colombo, Lenzi, Garcia-Gimeno, Bermudez-Silva, Rodriguez de Fonseca, Banin, Aledo, Baixeras, Sanz, Cuesta-Muñoz: "Opposite clinical phenotypes of glucokinase disease: Description of a novel activating mutation and contiguous inactivating mutations in human glucokinase (GCK) gene." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 12, pp. 1983-9, (2009) (PubMed).

Yoshida, Kato, Yokoi, Oguri, Watanabe, Metoki, Yoshida, Satoh, Aoyagi, Nishigaki, Nozawa, Yamada: "Association of genetic variants with chronic kidney disease in individuals with different lipid profiles." in: International journal of molecular medicine, Vol. 24, Issue 2, pp. 233-46, (2009) (PubMed).

Target Details for GCK

Target: GCK (Glucokinase (Hexokinase 4) (GCK))

Alternative Name: GCK (GCK Products)

Synonyms: FGQTL3 antibody, GK antibody, GLK antibody, HHF3 antibody, HK4 antibody, HKIV antibody, HXKP antibody, LGLK antibody, MODY2 antibody, Gk antibody, Gls006 antibody, GCK antibody, BA4487 antibody, glk antibody, hk4 antibody, hhf3 antibody, hkiv antibody, hxkp antibody, gck-A antibody, mody2 antibody, glucokinase antibody, GLUKA antibody, RNGK2 antibody, glucokinase antibody, glucokinase (hexokinase 4) L homeolog antibody, glucokinase (hexokinase 4) antibody, GCK antibody, Gck antibody, glcK antibody, Bphyt_1025 antibody, Halhy_6548 antibody, gk antibody, gck.L antibody, gck antibody

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Synonyms: GK, GLK, HK4, HHF3, HKIV, HXKP, LGLK, MODY2, FGQTL3

Molecular Weight: 52kDa

Gene ID: 2645

Pathways: MAPK Signaling, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process