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Glycogen Synthase Kinase 3 alpha (GSK3a) (pSer21) antibody

Antigen

Glycogen Synthase Kinase 3 alpha (GSK3a)
Synonyms GSK3A, 2700086H06Rik, MGC159759, DKFZp686D0638, CG5182, DmelCG5182, GSK3a, GSK3, GSK-3[a], GSK-3[b], xgsk3
Binding Site
Alternatives

pSer21
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
6 references available
Catalog no. ABIN966232
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name GSK3alpha
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human GSK3 around the phosphorylation site of serine 21 (T-S-SP-F-A).
Specificity GSK3 (phospho-Ser21) antibody detects endogenous levels of GSK3 only when phosphorylated at serine 21.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Pandey, Anand-Srivastava, Srivastava: "Vanadyl sulfate-stimulated glycogen synthesis is associated with activation of phosphatidylinositol 3-kinase and is independent of insulin receptor tyrosine phosphorylation." in: Biochemistry, Vol. 37, Issue 19, pp. 7006-14, 1998 (PubMed).

Srivastava, Pandey: "Potential mechanism(s) involved in the regulation of glycogen synthesis by insulin." in: Molecular and cellular biochemistry, Vol. 182, Issue 1-2, pp. 135-41, 1999 (PubMed).

Leadbetter: "Cultivation of recalcitrant microbes: cells are alive, well and revealing their secrets in the 21st century laboratory." in: Current opinion in microbiology, Vol. 6, Issue 3, pp. 274-81, 2003 (PubMed).

Koivisto, Alavian, Hakkinen et al.: "Glycogen synthase kinase-3 regulates formation of long lamellipodia in human keratinocytes." in: Journal of cell science, Vol. 116, Issue Pt 18, pp. 3749-60, 2003 (PubMed).

Barry, Graham, Fry et al.: "Regulation of glycogen synthase kinase 3 in human platelets: a possible role in platelet function?" in: FEBS letters, Vol. 553, Issue 1-2, pp. 173-8, 2003 (PubMed).

Welsh, Wilson, Proud: "GSK3: a SHAGGY frog story." in: Trends in cell biology, Vol. 6, Issue 7, pp. 274-9, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Glycogen Synthase Kinase 3 alpha (GSK3a)", type "Antibodies"
Hosts Rabbit (133), Mouse (54), Chicken (2)
Reactivities Human (178), Mouse (Murine) (115), Rat (Rattus) (113), Dog (Canine) (58), Pig (Porcine) (55), Chicken (40), Horse (Equine) (36), Cow (Bovine) (21), Monkey (5), Fruit Fly (Drosophila melanogaster) (2), Cat (Feline) (1), Hamster (1), Rabbit (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (133), Immunofluorescence (IF) (66), ELISA (64), Immunohistochemistry (IHC) (36), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunoprecipitation (IP) (31), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (9), Immunocytochemistry (ICC) (7), Immunoelectron Microscopy (IEM) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1)
Conjugates HRP (11), Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), Biotin (3), Cy3 (3), Cy5 (3), Cy5.5 (3), Cy7 (3), FITC (3), Gold (3), PE (3), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), Sepharose (3)
Epitopes pSer21 (61), C-Term (9), N-Term (5), Ser21 (3), AA 16-25 (2), pTyr279, pTyr216 (2), AA 1-156 (1), AA 16-25,pSer21 (1), AA 203-219 (1), AA 471-483 (1), AA 7-36 (1), AA 89-100 (1), C-Term,AA 347-376 (1), Phosphospecific (1), pSer9 (1)