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GST antibody (Glutathione S Transferase)

Details for Product anti-GST Antibody No. ABIN966238, Supplier: Log in to see
Antigen
  • GST
  • GST 13-13
  • GST13
  • GST13-13
  • GSTK1-1
  • hGSTK1
  • GSTS
  • GSTS1-1
  • PGD2
  • PGDS
  • H-PGDS
  • Ptgds2
  • 1500002K10Rik
  • Gst
  • glutathione S-transferase kappa 1
  • hematopoietic prostaglandin D synthase
  • microsomal glutathione S-transferase 1
  • GSTK1
  • HPGDS
  • Hpgds
  • Mgst1
Reactivity
Schistosoma japonicum
315
85
37
17
11
10
5
3
2
2
1
1
1
1
Host
207
161
99
5
3
2
Clonality
Monoclonal
Conjugate
This GST antibody is un-conjugated
32
31
26
15
8
7
6
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Western Blotting (WB)
373
219
92
90
41
30
17
12
11
10
9
9
9
8
7
6
5
3
3
2
2
2
1
1
1
1
1
1
Options
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Isotype IgG1
Specificity Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Purification Antibodies are purified by protein A affinity chromatography
Alternative Name Glutathione-S-Transferase (GST) (GST Antibody Abstract)
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Application Notes Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Storage -20 °C
Product cited in: Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, 1988 (PubMed).