HGF antibody (N-Term)

Catalog No. ABIN966282

Product Details anti-HGF Antibody

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hepatocyte growth factor ( HGF) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human HGF, different from the related mouse sequence by two amino acids.< br/>Immunogen was affinity purified.

Isotype: IgG

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.

References for anti-HGF antibody (ABIN966282)

Kilby, Afford, Li, Strain, Ahmed, Whittle: "Localisation of hepatocyte growth factor and its receptor (c-met) protein and mRNA in human term placenta." in: Growth factors (Chur, Switzerland), Vol. 13, Issue 1-2, pp. 133-9, (1996) (PubMed).

Tamura, Arakaki, Tsubouchi, Takada, Daikuhara: "Enhancement of human hepatocyte growth factor production by interleukin-1 alpha and -1 beta and tumor necrosis factor-alpha by fibroblasts in culture." in: The Journal of biological chemistry, Vol. 268, Issue 11, pp. 8140-5, (1993) (PubMed).

Gohda, Tsubouchi, Nakayama, Hirono, Sakiyama, Takahashi, Miyazaki, Hashimoto, Daikuhara: "Purification and partial characterization of hepatocyte growth factor from plasma of a patient with fulminant hepatic failure." in: The Journal of clinical investigation, Vol. 81, Issue 2, pp. 414-9, (1988) (PubMed).

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte growth factor (HGF Products)

Synonyms: DFNB39 antibody, F-TCF antibody, HGFB antibody, HPTA antibody, SF antibody, HGF antibody, C230052L06Rik antibody, HGF/SF antibody, NK1 antibody, NK2 antibody, SF/HGF antibody, hepatocyte growth factor antibody, HGF antibody, Hgf antibody

Background: Hepatocyte Growth Factor (HGF) is structurally similar, but not identical to scatter factor, a molecule shown to stimulate the dissociation and scattering of epithelial cells. It mapped to 7q21. 1 and involved in liver regeneration. Human HGF has been purified approximately 209,000-fold with 18 % yield from plasma of a patient with fulminant hepatic failure. It consists of 2 chains, heavy and light with molecular weights of 54,000-65,000 and 31,500-34,500, respectively. HGF is a potent mitogen, morphogen and motogen to both endothelial and epithelial cell types and is linked to a tyrosine kinase, proto-oncogene, c-met receptor. It indicated that HGF may serve as a paracrine mediator to control placental development and growth.

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor