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Histone H3.1 antibody (HIST1H3B) (pSer10)

Details for Product anti-HIST1H3B Antibody No. ABIN966291, Supplier: Log in to see
Antigen
  • H3 histone
  • H3-H
  • H3.1-221
  • H3.f
  • H3/f
  • H3/i
  • H3FF
  • H3FI
  • Hist1h2ai
  • M32460
Epitope
pSer10
37
18
10
7
6
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
117
47
47
2
2
1
1
1
Host
Rabbit
114
4
2
Conjugate
This Histone H3.1 antibody is un-conjugated
3
3
3
3
3
3
Application
Immunohistochemistry (IHC)
103
80
48
38
19
15
12
4
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).
Specificity Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Histone H3.1 (HIST1H3B Antibody Abstract)
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Dai, Sultan, Taylor, Higgins: "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment." in: Genes & development, Vol. 19, Issue 4, pp. 472-88, 2005 (PubMed).

Yih, Hsueh, Luu, Chiu, Lee: "Arsenite induces prominent mitotic arrest via inhibition of G2 checkpoint activation in CGL-2 cells." in: Carcinogenesis, Vol. 26, Issue 1, pp. 53-63, 2004 (PubMed).