Histone H3.1 (HIST1H3B) (pSer10) antibody

Antigen: Histone H3.1 (HIST1H3B)

Synonyms: H3/l, H3FL, HIST1H3A, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3/A, H3FA, HIST1H3B, H3.1, H3/c, H3FC, H3/d, H3FD, H3.f, H3/f, H3FF, H3/h, H3FH, H3/j, H3FJ, H3/k, H3FK, H3F1K, FLJ92264, H3/i, H3FI, M32460, H3.1-221, Hist1h2ai, H3 histone, H3-H, Hist1h3a, Hist1h3g, Hist1h3h, MGC144390, Hist1h3i, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, MGC89877

Binding Site: pSer10

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966291

Additional Information

Alternative name: Histone H3.1

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).

Specificity: Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.

Synonyms: H3/l, H3FL, HIST1H3A, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, H3/A, H3FA, HIST1H3B, H3.1, H3/c, H3FC, H3/d, H3FD, H3.f, H3/f, H3FF, H3/h, H3FH, H3/j, H3FJ, H3/k, H3FK, H3F1K, FLJ92264, H3/i, H3FI, M32460, H3.1-221, Hist1h2ai, H3 histone, H3-H, Hist1h3a, Hist1h3g, Hist1h3h, MGC144390, Hist1h3i, H3F3, H3.3A, H3F3B, MGC87782, MGC87783, H3.3B, H3F3A, h3f3a, MGC52829, MGC86731, MGC165454, zgc:56193, zgc:86731, wu:fb58e10, MGC89877

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Yih, Hsueh, Luu et al.: "Arsenite induces prominent mitotic arrest via inhibition of G2 checkpoint activation in CGL-2 cells." in: Carcinogenesis, Vol. 26, Issue 1, pp. 53-63, 2004 (PubMed).

Dai, Sultan, Taylor et al.: "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment." in: Genes & development, Vol. 19, Issue 4, pp. 472-88, 2005 (PubMed).