Histone H3.1 (HIST1H3B) (pSer10) antibody

Details for Product No. ABIN966291
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Antigen
Synonyms H3.f, H3/f, H3FF, H3-H, H3 histone, Hist1h2ai, H3/i, H3FI, H3.1-221, M32460
Epitope
pSer10
(32), (15), (5), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(90), (46), (43), (1), (1), (1), (1)
Host
Rabbit
(82), (9)
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC)
(82), (50), (43), (20), (14), (8)
Pubmed 2 references available
Quantity 100 μg
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Catalog No. ABIN966291
398.75 $
Plus shipping costs $45.00

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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Histone H3.1 around the phosphorylation site of serine 10 (R-K-SP-T-G).
Specificity Histone H3.1 (phospho-Ser10) antibody detects endogenous levels of Histone H3.1 only when phosphorylated at serine 10.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name Histone H3.1
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Yih, Hsueh, Luu et al.: "Arsenite induces prominent mitotic arrest via inhibition of G2 checkpoint activation in CGL-2 cells." in: Carcinogenesis, Vol. 26, Issue 1, pp. 53-63, 2004 (PubMed).

Dai, Sultan, Taylor et al.: "The kinase haspin is required for mitotic histone H3 Thr 3 phosphorylation and normal metaphase chromosome alignment." in: Genes & development, Vol. 19, Issue 4, pp. 472-88, 2005 (PubMed).

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