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Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (pSer32,pSer36) antibody

Antigen

Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)
Synonyms IKBA, MAD-3, NFKBI
Binding Site
Alternatives

pSer32,pSer36
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966363
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IkB-alpha
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IB- around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).
Specificity IB- (phospho-Ser32/Ser36) antibody detects endogenous levels of IB- only when phosphorylated at serine 32/36.

Application Details
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Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Honda, Tanaka, Yasuda: "Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53." in: FEBS letters, Vol. 420, Issue 1, pp. 25-7, 1998 (PubMed).

Tibbetts, Brumbaugh, Williams et al.: "A role for ATR in the DNA damage-induced phosphorylation of p53." in: Genes & development, Vol. 13, Issue 2, pp. 152-7, 1999 (PubMed).

Shieh, Taya, Prives: "DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization." in: The EMBO journal, Vol. 18, Issue 7, pp. 1815-23, 1999 (PubMed).

Hirao, Kong, Matsuoka et al.: "DNA damage-induced activation of p53 by the checkpoint kinase Chk2." in: Science (New York, N.Y.), Vol. 287, Issue 5459, pp. 1824-7, 2000 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA)", type "Antibodies"
Hosts Rabbit (196), Mouse (26), Sheep (4), Goat (1)
Reactivities Human (219), Mouse (Murine) (136), Rat (Rattus) (109), Cow (Bovine) (59), Pig (Porcine) (59), Sheep (Ovine) (54), Dog (Canine) (22), Chicken (20), Cat (Feline) (1), Hamster (1), Rabbit (1)
Applications Western Blotting (WB) (134), Immunofluorescence (IF) (85), ELISA (78), Immunohistochemistry (IHC) (62), Immunoprecipitation (IP) (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (39), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunocytochemistry (ICC) (9), Flow Cytometry (FACS) (5), Dot Blot (DB) (4), Immunoelectron Microscopy (IEM) (4), Gel Shift (GS) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Dot Blot (Dot) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Biotin (11), Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4)
Epitopes pTyr42 (28), pSer32,pSer36 (20), pTyr305 (19), pSer32, pSer36 (13), C-Term (8), N-Term (6), pSer36,pSer32 (5), AA 32-291 (4), Ser32, Ser36 (3), AA 19-49 (2), AA 34-48 (2), Pser32,pSer36 (2), Ser32 (2), Ser32,Ser36 (2), Tyr181 (2), Tyr42 (2), AA 130-143 (1), AA 18-48 (1), AA 20-50 (1), AA 32-36 (1), AA 32-47 (1), Internal Region (1), Ser23 (1), Sumoylation Site (1), pSer32 (1), pSer320 (1), pSer36 (1)