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NFKBIA antibody (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha) (pSer32)

Details for Product anti-NFKBIA Antibody No. ABIN966363, Supplier: Log in to see
Antigen
  • AI462015
  • Nfkbi
  • IKBA
  • MAD-3
  • NFKBI
  • ECI-6/IKBA
  • RL/IF-1
Alternatives
anti-Human NFKBIA antibody for Western Blotting
Epitope
pSer32, pSer36
51
48
36
29
24
23
22
15
14
13
13
13
7
5
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
370
173
137
17
12
8
6
4
2
1
Host
Rabbit
321
55
5
1
Conjugate
This NFKBIA antibody is un-conjugated
14
11
9
8
6
6
4
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC)
261
140
119
59
52
49
34
19
12
11
6
5
4
3
2
1
1
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IB- around the phosphorylation site of serine 32/36 (H-D-SP-G-L-D-SP -M-K).
Specificity IB- (phospho-Ser32/Ser36) antibody detects endogenous levels of IB- only when phosphorylated at serine 32/36.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name IkB-alpha (NFKBIA Antibody Abstract)
Pathways NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes IHC: 1:50-1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Hirao, Kong, Matsuoka et al.: "DNA damage-induced activation of p53 by the checkpoint kinase Chk2." in: Science (New York, N.Y.), Vol. 287, Issue 5459, pp. 1824-7, 2000 (PubMed).

Shieh, Taya, Prives: "DNA damage-inducible phosphorylation of p53 at N-terminal sites including a novel site, Ser20, requires tetramerization." in: The EMBO journal, Vol. 18, Issue 7, pp. 1815-23, 1999 (PubMed).

Tibbetts, Brumbaugh, Williams et al.: "A role for ATR in the DNA damage-induced phosphorylation of p53." in: Genes & development, Vol. 13, Issue 2, pp. 152-7, 1999 (PubMed).

Honda, Tanaka, Yasuda: "Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53." in: FEBS letters, Vol. 420, Issue 1, pp. 25-7, 1998 (PubMed).

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