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conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibody

Antigen

conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)
Synonyms IKK1, IKKA, IKBKA, TCF16, NFKBIKA, IKK-alpha, Ikka, Chuk1, Fbx24, Fbxo24, AI256658, MGC25325, CHUK, chuk, MGC83432, DKFZp459O053
Binding Site
Alternatives

pThr23
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN966382
Quantity 100ug  (100ug/100ul.)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IKK alpha
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IKK around the phosphorylation site of threonine 23 (L-G-TP-G-G).
Specificity IKK (phospho-Thr23) phosphorylated at threonine 23.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Ozes, Mayo, Gustin et al.: "NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase." in: Nature, Vol. 401, Issue 6748, pp. 82-5, 1999 (PubMed).

Yuan, Feldman, Sun et al.: "Inhibition of JNK by cellular stress- and tumor necrosis factor alpha-induced AKT2 through activation of the NF kappa B pathway in human epithelial Cells." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29973-82, 2002 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK)", type "Antibodies"
Hosts Rabbit (110), Mouse (28)
Reactivities Human (135), Mouse (Murine) (46), Rat (Rattus) (46), Dog (Canine) (33), Horse (Equine) (28), Chicken (5), Cow (Bovine) (5), Pig (Porcine) (5)
Applications Western Blotting (WB) (101), ELISA (59), Immunofluorescence (IF) (46), Immunohistochemistry (IHC) (21), Immunoprecipitation (IP) (21), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (19), Immunocytochemistry (ICC) (11), Flow Cytometry (FACS) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 488 (5), Alexa Fluor 350 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (3), Alexa Fluor (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pThr23 (17), AA 716-734 (8), AA 658-674 (6), C-Term (6), pTyr463 (5), AA 699-715 (4), pSer180,pSer176 (4), AA 557-745 (3), Thr23 (3), AA 500-590 (2), N-Term (2), Ser180, Ser181 (1), pSer176 (1), pSer176, pSer177 (1), pSer176, pSer180 (1), pSer31 (1), pTyr188 (1)