IL-6 (C-Term,AA 195-211) antibody

Antigen: IL-6

Binding Site: C-Term,AA 195-211

Clonality: Polyclonal

Host: Please enquire

Reactivity: Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN966395

Additional Information

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of rat IL-6 (195-211 aa), different from the mouse sequence by two amino acids.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Description: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Bowcock et al. (1988) assigned the IL6 gene to chromosome 7p21. By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al. (1988) mapped the IL6 gene to chromosome 7p15.

Application Details

Application Notes: WB: The detection limit for IL-6 is approximately 5ng/lane under non-reducing and reducing conditions.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative: 0.05mg Thiomersal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month.

Research Area: Cytokines, Immunology, Virology, Inflammation, Cancer, Receptors

Restrictions: For Research Use only

Publications

Product: Ferguson-Smith, Chen, Newman et al.: "Regional localization of the interferon-beta 2/B-cell stimulatory factor 2/hepatocyte stimulating factor gene to human chromosome 7p15-p21." in: Genomics, Vol. 2, Issue 3, pp. 203-8, 1988 (PubMed).