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Insulin Receptor Substrate 1 (IRS1) (pSer639) antibody

Antigen

Insulin Receptor Substrate 1 (IRS1)
Synonyms HIRS-1, G972R, IRS-1, IRS1IRM, irs1, MGC75645, IRS1
Binding Site
Alternatives

pSer639
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966415
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name IRS-1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human IRS-1 around the phosphorylation site of serine 639 (P-K-SP-V-S).
Specificity IRS-1 (phospho-Ser639) antibody detects endogenous levels of IRS-1 only when phosphorylated at serine 639.

Application Details
Publications Alternatives Variants back to top

Application Notes IHC: 1:50-1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Ozes, Akca, Mayo et al.: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Batty, Fleming, Downes: "Muscarinic-receptor-mediated inhibition of insulin-like growth factor-1 receptor-stimulated phosphoinositide 3-kinase signalling in 1321N1 astrocytoma cells." in: The Biochemical journal, Vol. 379, Issue Pt 3, pp. 641-51, 2004 (PubMed).

Steppan, Wang, Whiteman et al.: "Activation of SOCS-3 by resistin." in: Molecular and cellular biology, Vol. 25, Issue 4, pp. 1569-75, 2005 (PubMed).

Tzatsos, Kandror: "Nutrients suppress phosphatidylinositol 3-kinase/Akt signaling via raptor-dependent mTOR-mediated insulin receptor substrate 1 phosphorylation." in: Molecular and cellular biology, Vol. 26, Issue 1, pp. 63-76, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Insulin Receptor Substrate 1 (IRS1)", type "Antibodies"
Hosts Rabbit (256), Mouse (12)
Reactivities Human (251), Rat (Rattus) (182), Mouse (Murine) (180), Pig (Porcine) (68), Cow (Bovine) (51), Chicken (44), Dog (Canine) (41), Horse (Equine) (40), Guinea Pig (15), Sheep (Ovine) (10), Cat (Feline) (1)
Applications Western Blotting (WB) (138), Immunofluorescence (IF) (110), ELISA (72), Immunohistochemistry (IHC) (67), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunocytochemistry (ICC) (21), Immunoprecipitation (IP) (15), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (12), Alexa Fluor 488 (12), Alexa Fluor 555 (12), Alexa Fluor 647 (12), PE,Cy5.5 (12), Biotin (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer307 (15), pSer312 (13), pSer612 (10), pSer639 (10), Internal Region (9), pSer636 (9), C-Term (8), pSer1101 (6), pSer302 (6), pSer789 (6), pTyr896,C-Term (6), Ser639 (5), pSer336,pSer332 (5), pSer639,pSer636 (5), pTyr1222 (5), pThr176 (4), pTyr895 (4), pTyr896 (4), Ser636 (3), pSer312,Internal Region (3), pSer616 (3), pTyr1179 (3), pTyr1229 (2), pTyr941 (2), AA 1220-1235 (1), AA 1222-1235 (1), AA 1223-1242 (1), AA 298-316,pSer307 (1), AA 431-446 (1), AA 801-1020 (1), Middle Region (1), Ser312 (1), pSer259 (1), pSer323 (1), pSer472,pSer471 (1), pSer636, pSer639 (1), pSer794 (1), pThr1179 (1), pTyr449 (1), pTyr612 (1), pTyr989 (1)