Kelch-Like 22 (Drosophila) (KLHL22) antibody

Antigen: Kelch-Like 22 (Drosophila) (KLHL22)

Clonality: Monoclonal (7B10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN966459

Additional Information

Alternative name: KLHL22

Gene ID: 84861

Immunogen: Purified recombinant fragment of human KLHL22 expressed in E. Coli.

Isotype: IgG1

Clone: 7B10

Description: KLHL22 (kelch-like protein 22) is a 634 amino acid protein that is related to the Drosophilakelch protein, which is required to maintain Actin organization in ovarian ring canals. Mutations affecting Kelch function result in the failure of Kelch to associate with the ring canals and subsequent female sterility. Human KLHL22 protein contains six kelch repeats and one BTB (POZ) domain. The BTB (Broad-Complex, Tramtrack and Bric a brac) domain, also known as the POZ (Poxvirus and Zinc finger) domain, is an N-terminal homodimerization domain that contains multiple copies of kelch repeats and/or C2H2-type zinc fingers. Proteins that contain BTB domains are thought to be involved in transcriptional regulation via control of chromatin structure and function. There are two isoforms of KLHL22 that are produced as a result of alternative splicing events.
Synonyms: KELCHL, KLHL22

Molecular Weight: 72kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Restrictions: For Research Use only

Publications

Product: Maruyama, Sugano: "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides." in: Gene, Vol. 138, Issue 1-2, pp. 171-4, 1994 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Sowa, Bennett, Gygi et al.: "Defining the human deubiquitinating enzyme interaction landscape." in: Cell, Vol. 138, Issue 2, pp. 389-403, 2009 (PubMed).