KSHVORF62 antibody

Catalog No. ABIN966466

Product Details

Target: KSHVORF62

Reactivity: Human Herpesvirus 8 (HHV-8)

Host: Please inquire

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Ni-NTA purified recombinant human KSHV ORF62 expressed in E. Coli strain BL21 (DE3).

Purification: Crude ascites.

Isotype: IgM

Application Details

Application Notes: Dilution: Western Blot: 1:500-1,000
ELISA: Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Wu, Lo, Yu, Stoops, Forghani, Zhou: "Three-dimensional structure of the human herpesvirus 8 capsid." in: Journal of virology, Vol. 74, Issue 20, pp. 9646-54, (2000) (PubMed).

Russo, Bohenzky, Chien, Chen, Yan, Maddalena, Parry, Peruzzi, Edelman, Chang, Moore: "Nucleotide sequence of the Kaposi sarcoma-associated herpesvirus (HHV8)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 25, pp. 14862-7, (1997) (PubMed).

Chang, Cesarman, Pessin, Lee, Culpepper, Knowles, Moore: "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma." in: Science (New York, N.Y.), Vol. 266, Issue 5192, pp. 1865-9, (1995) (PubMed).

Target Details

Target: KSHVORF62

Target Type: Viral Protein

Background: Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi's sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman's disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. At least five major proteins are likely to be involved in the assembly of the HHV-8 capsid, including a protease (encoded by ORF17), the major capsid protein (encoded by ORF25), and three other smaller capsid proteins (encoded by ORF62, ORF26, and ORF65). Previous structural studies have shown that the HSV-1 triplex is a monomer of VP19c and a dimer of VP23 and that the HCMV triplex is similarly composed of a monomer and a dimer. By analogy, the KSHV triplexes are likely also composed of a monomer of the ORF62 protein and a dimer of the ORF26 protein, which are the respective homologs of VP19c and VP23.