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LIM Domain Kinase 1 antibody (LIMK1) (pThr508)

Details for Product anti-LIMK1 Antibody No. ABIN966484, Supplier: Log in to see
Antigen
  • limk1
  • xlimk1
  • GB16654
  • LIMK1
  • LIMK
  • LIMK-1
  • LIM domain kinase 1
  • LIM domain kinase 1a
  • LIM-kinase1
  • LIM-domain containing, protein kinase
  • LIMK1
  • limk1a
  • limk1
  • CpipJ_CPIJ000717
  • Limk1
Alternatives
anti-Human LIM Domain Kinase 1 antibody for Western Blotting
Epitope
pThr508
41
18
15
14
9
9
8
7
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
200
93
82
5
5
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
138
67
2
Conjugate
This LIM Domain Kinase 1 antibody is un-conjugated
6
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
166
78
62
54
36
27
17
13
12
5
4
2
1
1
Options
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
Specificity LIMK1 (phospho-Thr508) antibody detects endogenous levels of LIMK1 only when phosphorylated at threonine 508.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK1 (LIMK1 Antibody Abstract)
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Pathways Caspase Cascade in Apoptosis, Regulation of Cell Size
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Soosairajah, Maiti, Wiggan, Sarmiere, Moussi, Sarcevic, Sampath, Bamburg, Bernard: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, 2005 (PubMed).

Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch, Gill: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).