LIM Domain Kinase 1 antibody (LIMK1) (pThr508)

Details for Product anti-LIMK1 Antibody No. ABIN966484, Supplier: Log in to see
Antigen
  • limk1
  • xlimk1
  • GB16654
  • LIMK1
  • LIMK
  • LIMK-1
  • LIM domain kinase 1
  • LIM domain kinase 1a
  • LIM-kinase1
  • LIM-domain containing, protein kinase
  • LIMK1
  • limk1a
  • limk1
  • CpipJ_CPIJ000717
  • Limk1
Alternatives
anti-Human LIM Domain Kinase 1 antibody for Western Blotting
Epitope
pThr508
51
18
15
15
9
8
7
5
5
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
216
97
86
5
5
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
150
70
3
Conjugate
This LIM Domain Kinase 1 antibody is un-conjugated
7
7
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC)
182
84
66
56
39
27
17
16
13
6
4
2
1
1
Options
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
Specificity LIMK1 (phospho-Thr508) antibody detects endogenous levels of LIMK1 only when phosphorylated at threonine 508.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK1 (LIMK1 Antibody Abstract)
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Pathways Caspase Cascade in Apoptosis, Regulation of Cell Size, CXCR4-mediated Signaling Events
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Soosairajah, Maiti, Wiggan, Sarmiere, Moussi, Sarcevic, Sampath, Bamburg, Bernard: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, 2005 (PubMed).

Ohashi, Nagata, Maekawa, Ishizaki, Narumiya, Mizuno: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch, Gill: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).

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