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LIM Domain Kinase 1 (LIMK1) (pThr508) antibody

Antigen

LIM Domain Kinase 1 (LIMK1)
Synonyms LIMK, limk1, xlimk1, LIMK1, MGC146234, GB16654, DKFZp459P0831
Binding Site
Alternatives

pThr508
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966484
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name LIMK1
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK1 around the phosphorylation site of threonine 508 (R-Y-TP-V-V).
Specificity LIMK1 (phospho-Thr508) antibody detects endogenous levels of LIMK1 only when phosphorylated at threonine 508.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Neurology, Tyrosine Kinases, Cytoskeleton, Microfilaments
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Soosairajah, Maiti, Wiggan et al.: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "LIM Domain Kinase 1 (LIMK1)", type "Antibodies"
Hosts Rabbit (62), Mouse (18)
Reactivities Human (78), Rat (Rattus) (37), Mouse (Murine) (34), Cat (Feline) (1), Chicken (1), Chimpanzee (1), Cow (Bovine) (1), Dog (Canine) (1), Monkey (1)
Applications Western Blotting (WB) (62), ELISA (33), Immunofluorescence (IF) (33), Immunohistochemistry (IHC) (17), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (14), Flow Cytometry (FACS) (8), Immunoprecipitation (IP) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
Epitopes pThr508 (14), C-Term (4), N-Term (4), AA 630-647 (3), AA 548-648 (1), Internal Region (1), Thr508 (1), pTyr507, pThr508, pTyr504, pThr505 (1)