LIM Domain Kinase 2 (LIMK2) (pThr505) antibody

Antigen: LIM Domain Kinase 2 (LIMK2)

Synonyms: C85310, Limk2a, Limk2b, 9430059K01, A930024P04Rik, Link2, LIMK, xlimk2, zgc:91990, wu:fa97c09, wu:fk73e11, LIMK2, MGC134164

Binding Site: pThr505

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966486

Additional Information

Alternative name: LIMK2

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).

Specificity: LIMK2 (phospho-Thr505) antibody detects endogenous levels of LIMK2 only when phosphorylated at threonine 505.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Soosairajah, Maiti, Wiggan et al.: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, 2005 (PubMed).