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LIM Domain Kinase 2 (LIMK2) (pThr505) antibody

Details for Product No. ABIN966486, Supplier: Log in to see
Antigen
  • xlimk2
  • zgc:91990
  • wu:fa97c09
  • wu:fk73e11
  • LIMK2
  • Limk2b
  • Link2
  • LIMK
  • 9430059K01
  • A930024P04Rik
  • C85310
  • Limk2a
Epitope
pThr505
25
14
10
9
7
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
122
60
57
1
Host
Rabbit
110
7
3
2
Conjugate
Un-conjugated
1
Application
Immunohistochemistry (IHC)
106
51
44
37
11
8
2
1
1
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-TP-V-V).
Specificity LIMK2 (phospho-Thr505) antibody detects endogenous levels of LIMK2 only when phosphorylated at threonine 505.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name LIMK2 (LIMK2 Antibody Abstract)
Pathways
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Soosairajah, Maiti, Wiggan et al.: "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin." in: The EMBO journal, Vol. 24, Issue 3, pp. 473-86, 2005 (PubMed).

Ohashi, Nagata, Maekawa et al.: "Rho-associated kinase ROCK activates LIM-kinase 1 by phosphorylation at threonine 508 within the activation loop." in: The Journal of biological chemistry, Vol. 275, Issue 5, pp. 3577-82, 2000 (PubMed).

Edwards, Sanders, Bokoch et al.: "Activation of LIM-kinase by Pak1 couples Rac/Cdc42 GTPase signalling to actin cytoskeletal dynamics." in: Nature cell biology, Vol. 1, Issue 5, pp. 253-9, 1999 (PubMed).