Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3) (pSer189) antibody

Antigen

Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3)
Synonyms MEK3, MKK3, MAPKK3, PRKMK3, Prkmk3, mMKK3b, AW212142
Binding Site
Alternatives

pSer189
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
2 references available
Catalog no. ABIN966578
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name MKK3
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human MKK3 around the phosphorylation site of serine 189 (V-D-SP-V-A).
Specificity MKK3 (phospho-Ser189) Antibody detects endogenous levels of MKK3 only when phosphorylated at serine189.

Application Details
Publications Alternatives Variants back to top

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Research Area Kinases/Phosphatases
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Raingeaud, Whitmarsh, Barrett et al.: "MKK3- and MKK6-regulated gene expression is mediated by the p38 mitogen-activated protein kinase signal transduction pathway." in: Molecular and cellular biology, Vol. 16, Issue 3, pp. 1247-55, 1996 (PubMed).

Wang, Chen, Liao et al.: "Sequential activation of the MEK-extracellular signal-regulated kinase and MKK3/6-p38 mitogen-activated protein kinase pathways mediates oncogenic ras-induced premature senescence." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3389-403, 2002 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase Kinase 3 (MAP2K3)", type "Antibodies"
Hosts Rabbit (94), Mouse (6)
Reactivities Human (98), Rat (Rattus) (55), Mouse (Murine) (43), Dog (Canine) (4), Cow (Bovine) (3), Chicken (2), Zebrafish (2), Cat (Feline) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (77), ELISA (47), Immunofluorescence (IF) (35), Immunohistochemistry (IHC) (26), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunoprecipitation (IP) (8), Immunocytochemistry (ICC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (2), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer189 (11), Ser189 (6), N-Term (5), pThr222 (5), pSer189,Internal Region (3), Internal Region (2), C-Term (1), C-Term,AA 320-334 (1), Center (1), Ser222 (1)