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V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (pThr58) antibody

Antigen

V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)

Synonyms MRTL, c-Myc, bHLHe39, MYC, CMYC, MGC138120, c-myc, C-MYC, LOC100136746
Binding Site
Alternatives

pThr58

Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966616
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name Myc
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 58 (L-P-TP-P-P).
Specificity Myc (phospho-Thr58) antibody detects endogenous levels of Myc only when phosphorylated at threonine 58.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Baudino, Cleveland: "The Max network gone mad." in: Molecular and cellular biology, Vol. 21, Issue 3, pp. 691-702, 2001 (PubMed).

Welcker, Orian, Jin et al.: "The Fbw7 tumor suppressor regulates glycogen synthase kinase 3 phosphorylation-dependent c-Myc protein degradation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 24, pp. 9085-90, 2004 (PubMed).

Jin, Gao, Flagg et al.: "Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone promotes functional cooperation of Bcl2 and c-Myc through phosphorylation in regulating cell survival and proliferation." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 40209-19, 2004 (PubMed).

Abé: "Hormonal control of meiosis initiation in the testis from Japanese newt, Cynops pyrrhogaster." in: Zoological science, Vol. 21, Issue 7, pp. 691-704, 2004 (PubMed).

Alternatives

Alternatives for antigen "V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC)", type "Antibodies"
Hosts Rabbit (201), Mouse (89), Chicken (15), Goat (11), Sheep (3), Rat (1)
Reactivities Human (282), Rat (Rattus) (96), Mouse (Murine) (92), Dog (Canine) (30), Cow (Bovine) (28), Horse (Equine) (28), Pig (Porcine) (25), Rabbit (21), Sheep (Ovine) (18), Chicken (10), All Species (8), Cat (Feline) (4), Tag (4), Bird (Avian) (2), Virus (1)
Applications Western Blotting (WB) (204), ELISA (109), Immunofluorescence (IF) (99), Immunohistochemistry (IHC) (84), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (67), Immunoprecipitation (IP) (67), Flow Cytometry (FACS) (23), Immunocytochemistry (ICC) (21), Immunohistochemistry (Fixed) (IHC (fx)) (10), Electrophoretic Mobility-Shift Assay (EMSA) (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Dot Blot (Dot) (4), Immunoelectron Microscopy (IEM) (2), Transfected cell culture (TCC) (2)
Conjugates FITC (15), HRP (15), Biotin (9), Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE (3), PE,Cy5.5 (3), Peroxidase (POD) (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE-Cy3 (2), PE-Cy5 (2), PE-Cy5.5 (2), PE-Cy7 (2), Un-conjugated (2), Agarose Beads (1), Alkaline Phosphatase (AP) (1), RPE (1)
Epitopes C-Term (25), pThr373 (22), pThr358 (19), pSer373 (13), pThr58 (13), AA 410-419 (7), pSer62 (6), AA 408-439 (5), N-Term (5), Ser373 (5), Thr358 (4), Thr58 (4), pSer62,pThr58 (4), AA 408-420 (2), AA 408-439,C-Term (2), Ser62 (2), pThr58, pSer62 (2), AA 171-188 (1), C-Term,Thr400 (1), Thr58,Phosphospecific (1), pSer374 (1), pThr58,pSer62 (1), pTyr340,pSer338 (1)