NF-kB p100/p52 (pSer865) antibody

Antigen: NF-kB p100/p52

Binding Site: pSer865

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966651

Additional Information

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-B p100/p52 around the phosphorylation site of serine 865 (E-D-SP-A-Y).

Specificity: NF-B p100/p52 (phospho-ser865) antibody detects endogenous levels of NF-B p100/p52 only when phosphorylated at serine 865.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Xiao, Harhaj, Sun: "NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100." in: Molecular cell, Vol. 7, Issue 2, pp. 401-9, 2001 (PubMed).

Tang, Li, Pan et al.: "[River continuum characteristics of Xiangxi River]." in: Ying yong sheng tai xue bao = The journal of applied ecology / Zhongguo sheng tai xue xue hui, Zhongguo ke xue yuan Shenyang ying yong sheng tai yan jiu suo zhu ban, Vol. 15, Issue 1, pp. 141-4, 2004 (PubMed).

Qu, Qing, Rabson et al.: "Tax deregulation of NF-kappaB2 p100 processing involves both beta-TrCP-dependent and -independent mechanisms." in: The Journal of biological chemistry, Vol. 279, Issue 43, pp. 44563-72, 2004 (PubMed).