NF-kB p105/p50 (pSer337) antibody

Antigen: NF-kB p105/p50

Binding Site: pSer337

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966654

Additional Information

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-B p105/p50 around the phosphorylation site of serine 337 (R-K-SP-D-L).

Specificity: NF-B p105/p50 (phospho-Ser337) antibody detects endogenous levels of NF-B p105/p50 only when phosphorylated at serine 337.

Application Details

Application Notes: IHC: 1:50-1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Liu, Kuang, Gong et al.: "Principal component regression analysis with SPSS." in: Computer methods and programs in biomedicine, Vol. 71, Issue 2, pp. 141-7, 2003 (PubMed).

Hou, Guan, Ricciardi: "Phosphorylation of serine 337 of NF-kappaB p50 is critical for DNA binding." in: The Journal of biological chemistry, Vol. 278, Issue 46, pp. 45994-8, 2003 (PubMed).