p65 antibody (Nuclear Factor-KB P65) (pSer276)

Details for Product anti-NFkBP65 Antibody No. ABIN966657, Supplier: Log in to see
Antigen
  • NFKB3
  • p65
  • NFkB
  • v-rel avian reticuloendotheliosis viral oncogene homolog A
  • v-rel reticuloendotheliosis viral oncogene homolog A (avian)
  • RELA
  • Rela
Alternatives
anti-Human p65 antibody for Immunofluorescence (fixed cells)
Epitope
pSer276
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66
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31
24
21
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3
2
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2
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2
2
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2
2
2
2
1
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1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1179
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519
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31
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15
8
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Host
Rabbit
1011
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Conjugate
This p65 antibody is un-conjugated
34
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9
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4
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Application
Immunohistochemistry (IHC)
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427
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11
6
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2
2
1
Options
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of serine 276 (R-P-SP-D-R).
Specificity NF-B p65 (phospho-Ser276) antibody detects endogenous levels of NF-B p65 only when phosphorylated at serine 276.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Background publications Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

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