NFkB P65 (pSer536) antibody

Antigen: NFkB P65

Binding Site: pSer536

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966660

Additional Information

Alternative name: NF-kB p65

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).

Specificity: NF-B p65 (phospho-Ser536) antibody detects endogenous levels of NF-B p65 only when phosphorylated at serine 536.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Anwar, Fazal, Malik et al.: "RhoA/Rho-associated kinase pathway selectively regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via activation of I kappa B kinase beta and phosphorylation of RelA/p65." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6965-72, 2004 (PubMed).

Doyle, Jefferies, ONeill: "Bruton's tyrosine kinase is involved in p65-mediated transactivation and phosphorylation of p65 on serine 536 during NFkappaB activation by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23496-501, 2005 (PubMed).

Doyle, Monga, Montgomery et al.: "Arrhythmogenic right ventricular cardiomyopathy with implantable cardioverter defibrillator placement in pregnancy." in: The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, Vol. 18, Issue 2, pp. 141-4, 2005 (PubMed).

Gires, Klein, Baeuerle: "On the abundance of EpCAM on cancer stem cells." in: Nature reviews. Cancer, Vol. 9, Issue 2, pp. 143; author reply 143, 2009 (PubMed).