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p65 antibody (Nuclear Factor-KB P65) (pSer536)

Details for Product anti-NFkBP65 Antibody No. ABIN966660, Supplier: Log in to see
Antigen
  • NFkB
  • NFKB3
  • p65
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
pSer536
131
73
68
57
55
48
47
44
42
38
35
26
26
22
16
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3
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2
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2
2
2
2
2
2
2
2
2
1
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1
1
1
1
1
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1
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1
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1
1
1
1
1
1
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1
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1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1146
577
448
45
37
28
26
13
10
4
4
4
3
2
2
2
1
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Host
Rabbit
1020
122
13
10
7
Conjugate
This p65 antibody is un-conjugated
34
34
26
21
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11
9
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Application
Immunohistochemistry (IHC)
815
458
358
215
211
135
107
86
70
30
20
17
11
8
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6
6
4
4
2
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
Specificity NF-B p65 (phospho-Ser536) antibody detects endogenous levels of NF-B p65 only when phosphorylated at serine 536.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gires, Klein, Baeuerle: "On the abundance of EpCAM on cancer stem cells." in: Nature reviews. Cancer, Vol. 9, Issue 2, pp. 143; author reply 143, 2009 (PubMed).

Doyle, Monga, Montgomery, Dougherty: "Arrhythmogenic right ventricular cardiomyopathy with implantable cardioverter defibrillator placement in pregnancy." in: The journal of maternal-fetal & neonatal medicine, Vol. 18, Issue 2, pp. 141-4, 2005 (PubMed).

Anwar, Fazal, Malik, Rahman et al.: "RhoA/Rho-associated kinase pathway selectively regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via activation of I kappa B kinase beta and phosphorylation ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6965-72, 2004 (PubMed).

Background publications Doyle, Jefferies, ONeill: "Bruton's tyrosine kinase is involved in p65-mediated transactivation and phosphorylation of p65 on serine 536 during NFkappaB activation by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23496-501, 2005 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

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