You are viewing an incomplete version of our website. Please click to reload the website as full version.

p65 antibody (Nuclear Factor-KB P65) (pSer536)

Details for Product anti-NFkBP65 Antibody No. ABIN966660, Supplier: Log in to see
Antigen
  • NFkB
  • NFKB3
  • p65
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
pSer536
135
73
68
57
56
48
47
44
42
38
35
31
26
26
22
16
15
15
14
13
13
13
11
10
10
10
9
9
8
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1187
587
449
45
36
28
26
13
10
4
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
1043
138
15
10
7
Conjugate
This p65 antibody is un-conjugated
35
35
27
21
20
19
17
16
16
12
10
10
10
10
10
6
6
4
4
3
3
3
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (IHC)
842
470
369
224
220
135
119
90
71
30
19
17
11
8
7
6
6
4
4
2
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
Specificity NF-B p65 (phospho-Ser536) antibody detects endogenous levels of NF-B p65 only when phosphorylated at serine 536.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gires, Klein, Baeuerle: "On the abundance of EpCAM on cancer stem cells." in: Nature reviews. Cancer, Vol. 9, Issue 2, pp. 143; author reply 143, 2009 (PubMed).

Doyle, Monga, Montgomery, Dougherty: "Arrhythmogenic right ventricular cardiomyopathy with implantable cardioverter defibrillator placement in pregnancy." in: The journal of maternal-fetal & neonatal medicine, Vol. 18, Issue 2, pp. 141-4, 2005 (PubMed).

Anwar, Fazal, Malik, Rahman et al.: "RhoA/Rho-associated kinase pathway selectively regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via activation of I kappa B kinase beta and phosphorylation ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6965-72, 2004 (PubMed).

Background publications Doyle, Jefferies, ONeill: "Bruton's tyrosine kinase is involved in p65-mediated transactivation and phosphorylation of p65 on serine 536 during NFkappaB activation by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23496-501, 2005 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Did you look for something else?