anti-p65 antibody (Nuclear Factor-KB P65) (pSer536)

Details for Product anti-NFkBP65 Antibody No. ABIN966660
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Epitope
pSer536
(96), (74), (53), (47), (44), (42), (41), (38), (35), (34), (33), (32), (20), (13), (13), (13), (12), (12), (11), (8), (8), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(1087), (487), (400), (19), (12), (11), (8), (7), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(1003), (81), (15), (9), (2)
Conjugate
This p65 antibody is un-conjugated
(33), (31), (25), (20), (20), (20), (11), (9), (9), (9), (9), (9), (9), (9), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(862), (464), (400), (182), (176), (143), (91), (37), (27), (26), (25), (24), (20), (11), (7), (7), (5), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 100 μg
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of serine 536 (F-S-SP-I-A).
Specificity NF-B p65 (phospho-Ser536) antibody detects endogenous levels of NF-B p65 only when phosphorylated at serine 536.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Gires, Klein, Baeuerle: "On the abundance of EpCAM on cancer stem cells." in: Nature reviews. Cancer, Vol. 9, Issue 2, pp. 143; author reply 143, 2009 (PubMed).

Doyle, Monga, Montgomery et al.: "Arrhythmogenic right ventricular cardiomyopathy with implantable cardioverter defibrillator placement in pregnancy." in: The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, Vol. 18, Issue 2, pp. 141-4, 2005 (PubMed).

Doyle, Jefferies, ONeill: "Bruton's tyrosine kinase is involved in p65-mediated transactivation and phosphorylation of p65 on serine 536 during NFkappaB activation by lipopolysaccharide." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23496-501, 2005 (PubMed).

Anwar, Fazal, Malik et al.: "RhoA/Rho-associated kinase pathway selectively regulates thrombin-induced intercellular adhesion molecule-1 expression in endothelial cells via activation of I kappa B kinase beta and phosphorylation ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 11, pp. 6965-72, 2004 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Validation Images
Catalog No. ABIN966660
398.75 $
Plus shipping costs $45.00
Quantity
Price
100 μg
398.75 $

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