NFkB P65 (pThr254) antibody

Antigen: NFkB P65

Binding Site: pThr254

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966661

Additional Information

Alternative name: NF-kB p65

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).

Specificity: NF-B p65 (phospho-Thr254) antibody detects endogenous levels of NF-B p65 only when phosphorylated at threonine 254.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Concentration: 100ug/100ul.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Yeh, Yeh, Chuang et al.: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Zbar, Yeh: "Technical significance of reproducible rectoanal inhibitory reflexes." in: Techniques in coloproctology, Vol. 12, Issue 2, pp. 141-2, 2008 (PubMed).