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p65 antibody (Nuclear Factor-KB P65) (pThr254)

Details for Product anti-NFkBP65 Antibody No. ABIN966661, Supplier: Log in to see
Antigen
  • NFkB
  • NFKB3
  • p65
Alternatives
anti-Human p65 antibody for Western Blotting
Epitope
pThr254
135
74
68
57
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48
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35
31
26
26
22
16
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2
1
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1
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
1187
587
449
45
36
28
26
13
10
4
4
4
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2
2
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Host
Rabbit
1043
138
15
10
7
Conjugate
This p65 antibody is un-conjugated
35
35
27
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12
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6
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Application
Immunohistochemistry (IHC)
842
470
369
224
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135
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90
71
30
19
17
11
8
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6
4
4
2
2
1
1
Supplier
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).
Specificity NF-B p65 (phospho-Thr254) antibody detects endogenous levels of NF-B p65 only when phosphorylated at threonine 254.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Pathways NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Zbar, Yeh: "Technical significance of reproducible rectoanal inhibitory reflexes." in: Techniques in coloproctology, Vol. 12, Issue 2, pp. 141-2, 2008 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Background publications Yeh, Yeh, Chuang, Song, Cheng: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Haskill, Beg, Tompkins, Morris, Yurochko, Sampson-Johannes, Mondal, Ralph, Baldwin: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

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