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Nuclear Factor-KB P65 (NFkBP65) (pThr254) antibody

Details for Product No. ABIN966661
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Epitope
pThr254
(65), (54), (45), (44), (34), (33), (33), (30), (30), (29), (28), (19), (18), (12), (10), (7), (7), (7), (6), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(812), (413), (329), (76), (75), (74), (38), (8), (6), (3), (2), (2), (1), (1)
Host
Rabbit
(765), (59), (6), (5), (1)
Conjugate
Un-conjugated
(21), (18), (14), (11), (10), (10), (10), (10), (10), (10), (10), (10), (10), (10), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(620), (309), (297), (170), (121), (121), (100), (36), (21), (18), (12), (12), (7), (5), (2), (1)
Pubmed 5 references available
Quantity 100 µg
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Catalog No. ABIN966661
385.00 $
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).
Specificity NF-B p65 (phospho-Thr254) antibody detects endogenous levels of NF-B p65 only when phosphorylated at threonine 254.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Yeh, Yeh, Chuang et al.: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Zbar, Yeh: "Technical significance of reproducible rectoanal inhibitory reflexes." in: Techniques in coloproctology, Vol. 12, Issue 2, pp. 141-2, 2008 (PubMed).

Validation Images
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