anti-p65 antibody (Nuclear Factor-KB P65) (pThr254)

Details for Product anti-NFkBP65 Antibody No. ABIN966661
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Antigen
Synonyms c-Rel, C-Rel, Xrel2, c-rel, rel-A, v-rel, xrel, NFKB3, p65, NFkB, zgc:100833
Epitope
pThr254
(102), (80), (65), (46), (45), (45), (41), (39), (37), (34), (34), (33), (22), (14), (14), (14), (12), (12), (11), (9), (9), (8), (8), (7), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(1136), (497), (401), (19), (11), (11), (8), (7), (7), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(1043), (96), (16), (9), (8)
Conjugate
This p65 antibody is un-conjugated
(35), (34), (26), (24), (20), (20), (12), (10), (9), (9), (9), (9), (9), (9), (4), (2), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC)
(868), (465), (412), (189), (173), (140), (91), (41), (33), (29), (27), (24), (20), (11), (7), (6), (5), (4), (3), (2), (2), (2)
Pubmed 5 references available
Quantity 100 μg
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Immunogen The antiserum was produced against synthesized phosphopeptide derived from human NF-B p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P).
Specificity NF-B p65 (phospho-Thr254) antibody detects endogenous levels of NF-B p65 only when phosphorylated at threonine 254.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using nonphosphopeptide corresponding to the phosphorylation site.
Alternative Name NF-kB p65 (NFkBP65 Antibody Abstract)
Pathways NF-kappaB Signaling
Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Restrictions For Research Use only
Format Liquid
Concentration 100ug/100ul.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Product cited in: Zbar, Yeh: "Technical significance of reproducible rectoanal inhibitory reflexes." in: Techniques in coloproctology, Vol. 12, Issue 2, pp. 141-2, 2008 (PubMed).

Baeuerle, Baltimore: "NF-kappa B: ten years after." in: Cell, Vol. 87, Issue 1, pp. 13-20, 1996 (PubMed).

Background publications Yeh, Yeh, Chuang et al.: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Baeuerle, Henkel: "Function and activation of NF-kappa B in the immune system." in: Annual review of immunology, Vol. 12, pp. 141-79, 1994 (PubMed).

Haskill, Beg, Tompkins et al.: "Characterization of an immediate-early gene induced in adherent monocytes that encodes I kappa B-like activity." in: Cell, Vol. 65, Issue 7, pp. 1281-9, 1991 (PubMed).

Validation Images
Catalog No. ABIN966661
398.75 $
Plus shipping costs $45.00
Quantity
Price
100 μg
398.75 $

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