Lipocalin 2 (LCN2) (N-Term,AA 38-53) antibody

Antigen: Lipocalin 2 (LCN2)

Synonyms: 24p3, MSFI, NGAL, LCN2, Sip24, AW212229

Binding Site: N-Term,AA 38-53

Clonality: Polyclonal

Host: Please enquire

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN966671

Additional Information

Alternative name: NGAL

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of rat NGAL (38-53 aa), identical to the related mouse and rat sequence.

Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Description: Europhile gelatinase-associated lipocalin (NGAL) is a protein that in humans is encoded by the LCN2 gene. The binding of lipocalin-2 to bacterial siderophores is important in the innate immune response to bacterial infection. Upon encountering invading bacteria the toll-like receptors on immune cells stimulate the synthesis and secretion of lipocalin-2. Secreted lipocalin-2 then limits bacterial growth by sequestering iron-containing siderophores. Lipocalin-2 also functions as a growth factor.

Application Details

Application Notes: WB: The detection limit for NGAL is approximately 2.5ng/lane under non-reducing and reducing conditions.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative: 0.05mg Thiomersal, 0.05mg NaN3.

Storage: At -20°C for one year. After reconstitution, at 4°C for one month.

Restrictions: For Research Use only

Publications

Product: Kjeldsen, Johnsen, Sengeløv et al.: "Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase." in: The Journal of biological chemistry, Vol. 268, Issue 14, pp. 10425-32, 1993 (PubMed).

Chan, Simon-Chazottes, Mattei et al.: "Comparative mapping of lipocalin genes in human and mouse: the four genes for complement C8 gamma chain, prostaglandin-D-synthase, oncogene-24p3, and progestagen-associated endometrial protein map to HSA9 and MMU2." in: Genomics, Vol. 23, Issue 1, pp. 145-50, 1995 (PubMed).

Begley, Robb, Rockman et al.: "Structure of the gene encoding the murine SCL protein." in: Gene, Vol. 138, Issue 1-2, pp. 93-9, 1994 (PubMed).

Cowland, Borregaard: "Molecular characterization and pattern of tissue expression of the gene for neutrophil gelatinase-associated lipocalin from humans." in: Genomics, Vol. 45, Issue 1, pp. 17-23, 1997 (PubMed).

Matloubian, Lo, Cinamon et al.: "Lymphocyte egress from thymus and peripheral lymphoid organs is dependent on S1P receptor 1." in: Nature, Vol. 427, Issue 6972, pp. 355-60, 2004 (PubMed).

Flo, Smith, Sato et al.: "Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron." in: Nature, Vol. 432, Issue 7019, pp. 917-21, 2004 (PubMed).